Determining which organic molecules are present in a series of unknown dietary supplements ● Introduction:
Our main objective was to find out if the dietary supplements that claimed to be providing high levels of proteins contained fillers such as starch and sugar. We developed and carried out tests to determine which organic molecules were present in a series of unknown supplements. This is important because it is important to know what is in the food you eat for yourself and not always reply on the vague claims that companies make about their products.
Knowing that sugars react to a Benedict’s reagent, proteins will react to a Biuret reagent, and starch will react to a Lugol’s reagent, we will be able to test the presence …show more content…
For all three reactions, cover each test tube with parafilm after adding the reagent to mix the solution and the reagent together before observing the reaction.
To negatively test each solution, we obtained 5 mL of each stock solution
(protein, sugar, and starch) and added 1 mL of water in each solution and observed and noted each reaction.
To test which macromolecules are in each of the three unknown stock solution, obtain 6, 5 mL sample of each unknown stock solution(3 for each trial). You should have
18 solution samples in total. Label which tubes correspond to which unknown. First, place 1 mL of the Lugol reagent in two of the samples of each unknown. Cover the test tubes with para film and mix well. Observe the color change and report the results. Next, place 2 mL of Biuret reagent in two of the samples of each unknown. Cover the test tubes with para film and mix well. Observe the color change and report the results.
Lastly, place 1 mL of Benedict’s reagent in two of the sample of each unknown. Heat
Benedict’s reaction to 65 degrees celsius for 4-5 minutes for full color change. Cover the test tubes with para film and mix well. Observe the color change and report the …show more content…
From the comparison, decipher how prominent is each macromolecule is in each unknown based on pigmentation.
After running through the experiment, we concluded that unknown solution 1 contains starch since it reacted in a similar way in comparison to the positive control.
Also, we concluded that there are traces of sugar since it also reacted to the Benedict’s solution, but not as strongly as it did with the positive control. In unknown solution 2,