Determination of Copper(Ii) Concentration by Spectrophotometry
QUANTITATIVE DETERMINATION OF COPPER (II) CONCENTRATION BY SPECTROPHOTOMETRY
D.DEL PRADO1, J. BELANO1, M.MAHUSAY2,and M.FRANCISCO2
1 DEPARTMENT OF FOOD SCIENCE AND NUTRITION, COLLEGE OF HOME ECONOMICS
2INSTITUTE OF CHEMISTRY, COLLEGE OF SCIENCE
UNIVERSITY OF THE PHLIPPINES, DILIMAN, QUEZON CITY 1101, PHILIPPINES
DATE SUBMITTED: 12 MARCH 2013
DATE PERFORMED: 7 MARCH 2013
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ABSTRACT
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------------------------------------------------- Spectrophotometry has various uses in different fields of science. It can be used in obtaining the concentration and the identity of a solution. In order to test that, an experiment was made using the basic concepts of spectrophotometry. This experiment used the properties of light and how much of it is absorbed by the unknown solution. In relation to this method was the so called principle, the Beer-Lambert’s Law, which was used in getting the relationship of the absorbance and the concentration with one another. Then from the data gathered absorbance and concentration, a calibration curve was made and the equation of the line was determined. The absorbance of an aliquot of unknown copper-ammonia complex solution was then gathered in triplicate and its concentration was determined by the use of the equation determined earlier. The mean absorbance and concentration obtained were 0.2633 and 342.33 ppm, respectively. Using the aliquot factor, the calculated concentration of the unknown solution was 1,711.65 ppm. It was then concluded that this method of analysis was practical in identifying the concentration of Cu(II) solution.
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INTRODUCTION
The goals of the experiment were to apply the spectrophotometry in the quantitative analysis of copper (II) solutions, operate a spectrophotometer and measure transmission properties of solutions, and
D.DEL PRADO1, J. BELANO1, M.MAHUSAY2,and M.FRANCISCO2
1 DEPARTMENT OF FOOD SCIENCE AND NUTRITION, COLLEGE OF HOME ECONOMICS
2INSTITUTE OF CHEMISTRY, COLLEGE OF SCIENCE
UNIVERSITY OF THE PHLIPPINES, DILIMAN, QUEZON CITY 1101, PHILIPPINES
DATE SUBMITTED: 12 MARCH 2013
DATE PERFORMED: 7 MARCH 2013
-------------------------------------------------
-------------------------------------------------
ABSTRACT
-------------------------------------------------
------------------------------------------------- Spectrophotometry has various uses in different fields of science. It can be used in obtaining the concentration and the identity of a solution. In order to test that, an experiment was made using the basic concepts of spectrophotometry. This experiment used the properties of light and how much of it is absorbed by the unknown solution. In relation to this method was the so called principle, the Beer-Lambert’s Law, which was used in getting the relationship of the absorbance and the concentration with one another. Then from the data gathered absorbance and concentration, a calibration curve was made and the equation of the line was determined. The absorbance of an aliquot of unknown copper-ammonia complex solution was then gathered in triplicate and its concentration was determined by the use of the equation determined earlier. The mean absorbance and concentration obtained were 0.2633 and 342.33 ppm, respectively. Using the aliquot factor, the calculated concentration of the unknown solution was 1,711.65 ppm. It was then concluded that this method of analysis was practical in identifying the concentration of Cu(II) solution.
-------------------------------------------------
INTRODUCTION
The goals of the experiment were to apply the spectrophotometry in the quantitative analysis of copper (II) solutions, operate a spectrophotometer and measure transmission properties of solutions, and
References: [1]Sharma, S., et al.; "UV Spectrophotometric Determination of Cu(II) in Synthetic Mixture and Water Samples." Journal of the Chinese Chemical Society 57.4A (2010): 622-631. Web. 13 Mar. 2013. [2] Vo, Kevin. “Spectrophotometry-ChemWiki”. ChemWiki: The Dynamic Chemisty. N.p.Web. 11 Mar. 2013. [3] Gilreath, E.S [6] Gilreath, E.S. Elementary Quantitative Chemistry. 1969. San Francisco: W.H. Freeman and Company. 95 [7] Skoog, D.; West, D.; Holler F.J.; Crouch, S.R [9] Skoog, D.; West, D.; Holler F.J.; Crouch, S.R. Fundamentals of Analytical Chemistry. 8th Ed. 2004: Thompson Brooks/Cole. 751 [10] Harvey, D., Modern Analytical Chemistry [11] Skoog, D.; West, D.; Holler F.J.; Crouch, S.R. Fundamentals of Analytical Chemistry. 8th Ed. 2004: Thompson Brooks/Cole. 751 [12] Willard, H.H.; Furman, N.H [13] Skoog, D.; West, D.; Holler F.J.; Crouch, S.R. Fundamentals of Analytical Chemistry. 8th Ed. 2004: Thompson Brooks/Cole.