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Crayfish Lab Report

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Crayfish Lab Report
Introduction:

Crayfish also known as Procambarus clariia are classified as a decapod crustaceans

related to lobsters, crabs, and shrimp. They breathe through the gills as they are aquatic

animals but they can survive for short time outside the water. They have a hard, calcified

exoskeleton that must be shed as they grow. In front of the first abdominal segment

locates crayfish heart in the dorsal portion of cephalothorax.

Crayfish are poikilothermic as their internal temperature is proportional and similar to its

surroundings. Thus, poikilothermic animal metabolic rate and their other physiological

processes associate to its environmental temperature. Crayfish have an open circulatory

system and neurogenic hearts due to which
…show more content…
The temperature of water was determined by a thermometer. 1 cm

stripped ends of the wires was hooked into the input posts on the back of the

impendence converter. Lab tutor module was started after.

B. Baseline heart rate

The top of the container was covered with foil and the animal was allowed to sit

quietly for 10 min. The trace was examined by pressing start. After that the baseline

or the controlled heart rate was record for 5 to 10 minutes. After clicking stop the

heart rate was marked as the start and end of the baseline heart rate was traced.

C. Stressed Heart Rate

The container was uncovered and the trace was started. After that the trace was

examined and heart rate was recorded for 5 to 10 minutes. The start and end of the

stressed heart rate trace was marked after it was stopped.

D. Serotonin

1 mM Serotonin was obtained from the refrigerator. The micropipette with a blue

tip was used to transfer 1 ml of solution to the container. Final concentration of the

holding water was calculated of the neurotransmitter. The animal was allowed to sit

with the foil cover on for 5 minutes so that the neurotransmitter can equilibrate

between the holding water and the animal’s hemolymph. The trace was started
…show more content…
The error might have occurred while picking up the highest

stable point in the trace mark. It might also have happened because of the crayfish

being moved constantly while taking the temperature and it was stressed. But

however the result should have been the opposite and the hypothesis should have

been correct. Previous studies on poikilothermic organisms such as Daphnia magna

show a similar relationship in which increased temperature is proportional to

metabolic rate, heart rate, and other physiological processes (Khan, 2008). This may

be due to an increase in enzyme activity which is very influential in metabolic

processes. Since enzyme activity is increased with higher temperatures, more

metabolic processes are able to occur at a faster rate; thus, leading an increase in

heart rate. However our result doesn’t support the hypothesis that increased

temperatures lead to a higher heart rate or vice versa.

The second factor of the experiment was the stress level. For this the crayfish

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