Conclusions of Dh5Α Dna Transformation with Red Colonies Resistance to Ampicillin and the Lacz Gene

Topics: Bacteria, Escherichia coli, Agar plate Pages: 4 (1024 words) Published: March 14, 2012
Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene

In this experiment, a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin, and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed by a bacteria to improve the lives of people. Transformation happened when a gene is transferred from one bacterium to another one on a plasmid. E. coli is the organism that genetics prefer to use because it can be easily grown and suspended in LB broth. Escherichia coli, commonly abbreviated as “E. coli”, is a Gram-negative and rod-shaped bacterium. Usually found in the lower intestines of warm-blooded creatures, E. coli is generally non-lethal in most known strains. The more harmless strains are normal flora of the gut, performing vital and/or helpful tasks, such as the production of vitamin K, and preventing the establishment of pathogenic bacteria within the intestinal tract of the host body. E. coli, despite misconceptions, are actually often more helpful than harmful, and only make up approximately 0.1% of the gut flora. The strain used for the experiment was DH5α. The cells that take up this plasmid will show resistance to the antibiotic and produce a color change (dark red) as the lacZ gene converts lactose in the media. One of the most common mediums is an agar,. Lysogeny Broth, abbreviated through the rest of the report as “LB broth”, is a common medium, which is a light yellow in color. In this lab it changes to a red color in two of the dishes. It contains various nutrients that aid in the growth of bacteria such as E. coli, helping develop, and increase colonization of the bacteria. In its natural form, before being mixed with agar, it is a...
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