In part A of this experiment, we transformed the bacteria into an antibiotic resistant form by inserting a plasmid into it. We used heat shock in order to make the bacteria capable to uptake a plasmid in the presence of calcium ions that help disrupt the cell membrane (heat shock is the combination of altering hot and cold). When they are capable of accepting plasmids, the bacteria are incubated with plasmids that carry the resistance to a particular antibiotic, in this case ampicilin. We also ran a control sample along with the experimental one, which is treated the same way except it is not exposed to the plasmid. We then poured the cells onto four Petri dishes that contain Luria broth. Two of the Petri dishes contained the antibiotic, and the other two did not.
After completing part A, we found the first Petri dish, which had the antibiotic and plasmid, to have grown colonies. The second Petri dish, which had plasmids but no antibiotic, also had positive results. The third Petri dish had no plasmid but did contain the antibiotic. No growth occurred in this Petri dish. The last Petri dish had no plasmid and no antibiotic. The results were positive and growth was observed. In Petri dish 1, we found that colonies had formed. Each colony consists of bacteria that have been transformed and are resistant to the antibiotic because they received the plasmid. This Petri dish did glow when under the black light. Petri dish 2 had a lawn of bacteria. It experienced lawn growth because any bacteria would be able to grow on it due of the fact that no antibiotic is present to prevent it. Because it had the plasmid, it did glow when under the black light. Petri dish 3 (the control) had no growth. These bacteria contained the antibiotic and, because they were not incubated with the plasmid resistant to the antibiotic, the antibiotic was able to kill the bacteria. Petri dish 4 had lawn growth because it was incubated with plasmid and no antibiotic to kill the...
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