Isolation of antibacterial protein from coelomic fluid of earthworm-Perionyx excavatus ( Indian blues) and elucidation of its cytotoxic effects
3. PROJECT TITLE: “Isolation of antibacterial protein from coelomic fluid of earthworm - Perionyx excavatus ( Indian blues) and elucidation of its cytotoxic effects”
4. SPECIFIC AREA OF THE PROJECT: “ VERMITECHNOLOGY”
5.1 ORIGIN OF THE PROPOSAL:
Earthworms have long been known as Farmer’s friends because of their enormous use in agriculture. This coupled with the fact that it has been used in traditional medicine for 1000’s of years ( Prakash et al, 2008) has helped its research to reach new heights. Several drugs based on genomic approaches are in the market. Probably, earthworms are the most promising sources to combat various physiological and pathological disorders in humans. If the antibacterial protein is isolated and is proved non-cytotoxic to humans, then it can be researched and developed further as a drug for curing various diseases caused by Escherichia coli and Staphylococcus aureus ailments in human beings and thus give an impetus to further research on being used as model organisms to be studied.
5.2 DEFINITION OF THE PROBLEM:
Treating every bacterial-caused disease with antibiotics causes several side effects and in the long run antibiotic resistance towards that disease.
Hence to overcome this problem, I have chosen the protein that would be isolated from coelomic fluid of earthworm - Perionyx excavatus. This if proved non-cytotoxic to humans can be researched further on its antibacterial nature specific for Escherichia coli and Staphylococcus aureus.
5.3 SPECIFIC OBJECTIVE:
❖ Confirmation of antibacterial nature of proteins isolated from Perionyx excavatus towards Escherichia coli and Staphylococcus aureus.
❖ To determine non-cytotoxic nature of proteins isolated from Perionyx excavatus towards human beings.
❖ To analyse the proteins with insilico method.
6. BRIEF REVIEW OF CURRENT STATUS OF RESEARCH:
1. INTERNATIONAL STATUS:
Tram Thi Bich Phan et al (2011) have purified and characterized novel fibrinolytic proteases as potential antithrombotic agents from Perionyx excavatus.
Jessica Trisina et al ( 2011) have reported a protein extract from Lumbricus rubellus having antithrombotic and thrombolytic activities.
Hidehiko Shogomori et al ( 2007) have reported Lysenin, a sphingomyelin specific pore-forming toxin in Eisenia foetida.
2. NATIONAL STATUS:
Abhishek Mathur et al (2011) have reported anti-inflammatory activity of earthworm extracts.
Subathra S et al (2011) have reported fibrinolytic activity of serine protease of Perionyx excavatus.
Prakash et al (2007) have reported anti-ulceral and anti-oxidative properties of “earthworm paste” of Lampito mauritii (Kinberg) on albino rats Rattus Norvegicus.
6.3 IMPORTANCE OF THE PROPOSED PROJECT IN THE CONTEXT OF CURRENT STATUS
❖ The proposed projects results would help to minimize the antibiotic resistance in diseases caused by Escherichia coli and Staphylococcus aureus affected patients.
❖ If the experiment shows positive result (i.e. non-cytotoxic effect) the protein can be further researched as having a potential in drug research.
❖ If the experiment shows negative result ( i.e. cytotoxic effect) then it has to be researched further to convert it into a form so as to be useful as a drug.
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