Introduction Enteric clostridial infection in dogs are life-threatening condition usually caused by Clostridium perfringens and Clostridium difficile. The pathogenesis of enteric clostridial infection requires two factors; the presence of these microorganisms in the intestine and a change in the enteric microenvironment. C. perfringens is a Gram-positive, spore-forming, obligately anaerobic, non-motile bacillus and capable of toxin production.
Materials and methods
Case history: A male Maltese dog, weighing 2.04 kg, was died on the way to the animal hospital with clinical signs of weakness, large volume of bloody diarrhea but no vomit. The previous day the …show more content…
Many necrotic enterocytes, characterized by karyorrhexis and karyolysis, were seen and there were moderate numbers of lymphocytes and plasma cells infiltrated at the lamina propria and some areas of the intestinal submucosal layer. The serosal neurons were dark and swollen. The spleen showed a focal fibrosiderotic plaque and occasionally fibrin thrombi in the blood vessels. The kidney revealed mild multifocal necrosis of the tubular epithelial cells with diffuse congestion and multifocal cysts at the cortex. The liver sinusoids were markedly expanded by blood. The alveolar capillaries were markedly expanded by red blood cells and increased number of neutrophils and some fibrin thrombi were seen. Figure…
Clinical pathology: There were all negative on antigen rapid canine parvovirus/canine coronavirus (CPV/CCV) antigen test kit. The sample from small intestine was submitted for bacterial culture.
Bacterial cultures: a bacterial culture from intestinal sample isolated large numbers of C. perfringens on ….. agar plates incubated at 37◦C overnight in anaerobic conditions.
Figure… Colonies on blood agar, which are surrounded by a double zone of