Chromatography (Greek for ‘colour writing’) is used to describe various methods applied to separate mixtures (referred to as the sample of the experiment) with great accuracy to analyze them. By using chromatography we can manipulate these to move at different speeds through the system, thus separating them. Chromatography is necessary in chemical industries, as well as bio processing companies. Chromatography can be:
analytical: used to measure ratios of analytes(substance in simpler forms) in a mixture. Example- analyte 1: analyte 2 = 1:2 2.
preparative: for studying on a higher level
There are three main components of which a chromatographic experiment consists of, these are the: 1.
Mobile Phase: solvent into which sample is dissolved and helps sample travel through stationary phase. It is mobile. 2.
Stationary Phase: Permanent part of chromatographic procedure, through which the mobile phase moves. It is stationary i.e. it doesn’t move. 3.
Sample: This is the solution that has to be examined
Chromatography is a universal tool, which we can use to separate even the most similar substances, volatile or soluble, but only if you have the correct: 1.
Mikhail Tsvet (Russian Scientist)
Idea is first conceived
Mainly separated plant pigments (chlorophyll, carotenes and xanthophylls). these each were coloured differently, thus retaining the name chromatography 1930-
Technique became more and more globalised
Archer John Porter and Richard Laurence Millington Synge
Developed rules and methods to be followed out during process
Their work lead to the establishment of: gas, paper and high performance liquid chromatography
W C Still
Introduces Flash Column Chromatography (faster more efficient version of column chromatography)
Separation of mixtures into analytes can be performed within 20 minutes and is sold on mass...
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