Chemistry Project Report: To Study the Digestion of Starch by Salivary Amylase and Effect of pH and Temperature on it

Topics: Starch, Enzyme, Saliva Pages: 5 (950 words) Published: December 4, 2013
Chemistry Project Report on
To Study the Digestion of Starch by Salivary Amylase and Effect of pH and Temperature on it  
Submitted by :
Class XII (CBSE)
Sr. Sec. School
 
 

Contents
1. Certificate
2. Acknowledgement
3. Verification
4. Objectives of the Project
5. Introduction
6. Material Required
7. Procedure
8. Observation
9. Conclusion
10. Bibliography
 

 
Certificate
This is to certify that the investigatory Chemistry project report entitled “To Study the digestion of starch by salivary amylase and effect of temperature and pH on it” has been completed under my supervision.

 
Authorized Signatory
Chemistry Deptt.
 

 
Acknowledgement
As a student of class XII, I did this project as a part of my studies entitled “To Study the digestion of starch by salivary amylase and effect of temperature and pH on it” I owe a deep sense of gratitude to my chemistry teacher whose valuable advice, guidance help me in doing this project from conception to completion. At the same time I can't forget to express my thankfulness to Principal of our School for extending his generous patronage and constant encouragement. Finally, I am thankful to my parents for helping me economically and my friends for giving me a helping hand at every step of the project.  

(Signature of Student)
 

Objectives of the Project Report
The main objective of this chemistry project report is  “To Study the digestion of starch by salivary amylase and effect of temperature and pH on it” and To study digestion of starch by saliva.
To study the effect of temperature on the digestion of starch by saliva. To study the effect of pH on the salivary digestion of starch.  

Introduction
Every health book insists on the chewing of food. The act of chewing stimulates the excretion of saliva. Saliva mixes up with the food and helps its digestion. That is, the enzyme ptyalin or amylase present in human saliva hydrolyse the big molecules of food into many molecules. For example starch into mono-saccharides maltose and glucose; proteins into amino acids and fats into fatty acids and glycerol. Thus saliva not only helps in digestion of food but convert it into energy generating substances. Further, enzymes and their activity are very sensitive to temperature and pH. Even a slight variation in these two factors, can disrupt the action of enzymes. In other words, digestion of food by salivary amylase is also effected by pH and temperature and can be verified experimentally. For example, hydrolysis of starch can be verified by testing it with iodine solution. Starch forms blue coloured complex with iodine. If no starch is present in a system it will not give blue colour with iodine.  

Requirements for Chemistry Project Report
The requirements for experiment of Chemistry Project Report are as under : Test tubes
Test tube stand
One dropper
Beaker
Stop watch
Starch and Iodine solution
Thermometer
Dil. HCl and Dil NaOH solution.
 

Chemistry Experiment Procedure
Procedure for Chemistry Experiment is :
1) Collection of Saliva - Rinse mouth throughly with cold water and ensure that it does not contain any food particles. Now take about 20ml of lupe warm water in the mouth and gangle for about three minutes so that saliva mixes up well with it. Spit this into a beaker. Filter, if there is any suspended impurity clear filtrate is saliva solution and contains enzyme ptyalin. 2) Preparation of starch solution - Take about 0.5g of starch in a 100ml beaker and add enough water to make a paste. Dilute the paste by adding 50ml water and boil for about 5 min. 3) Digestion of starch (a) take 5ml of the starch solution in a test tube. Add 2 ml of saliva solution into it. Mix the solutions well by shaking the tube carefully and start a step watch. (b) After one minute take out two drops of the mixture solution from the test tube with the help of a dropper and transfer it into another test...
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