Chemistry Experiment Instructions

Satisfactory Essays
Procedure.
1) Accurately weigh approximately 2 g of the mix and dissolve it in 50 mL of diethyl ether. Be sure to dissolve the entire mix since it is not homogeneous.
2) Pour the solution into a separatory funnel.
3) To extract the nicotinamide from the mixture, add 5 mL of 5% HCl and shake gently. Draw off the lower aqueous layer into 125 mL Erlenmeyer flask and repeat the extraction with a second portion of HCl. Combine the three extracts and then set this mixture aside.
4) To separate the trans-cinnamic acid from the mixture, extract the ether solution twice with 5 mL of 5% sodium bicarbonate. Combine the extracts and set aside.
5) After neutralizing the aqueous solutions, filter or back extract with dichloromethane to obtain the separate compounds.
6) Pour out the remaining ether solution (which should contain only the 4-phenylphenol) through the neck of the separator funnel into a small Erlenmeyer flask. If any water droplets are visible, remove them before adding the drying agent. Add a small amount of anhydrous sodium sulfate (or magnesium sulfate) to the flask. Let the mixture stand for 5 min. occasionally swirling it gently.
7) Decant or pipette the organic liquid away from the drying agent and place it in a clean, dry Erlenmeyer flask. Place it in the hood to allow the ether to evaporate (if needed, set aside overnight to dry).
8) After separating the mix, collect the products by vacuum filtration. Wash with small amounts of distilled water.
9) Determine the mass and the identity of the major products by melting point.
10) Run a TLC for the qualitative analysis of the reaction products.

You May Also Find These Documents Helpful

  • Good Essays

    Chemistry Experiment

    • 1212 Words
    • 5 Pages

    General Chemistry I CHEM XXI Project Report Worksheet (4 pages max.) Experiment title: How Do We Identify an Unknown Substance? Guiding questions: State the question or questions that that you were trying to answer in this lab. How do you determine the two unknown types of plastic? What are the two unknown plastics? Safety considerations: Briefly describe the safety measures you had to take to perform the experiments. Before entering the lab, I put on my…

    • 1212 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Chemistry Experiment

    • 3949 Words
    • 16 Pages

    EXPERIMENT NO. 4 Chemical Equilibrium Claver, L. Z. X. Y.1, Palad, C. C.2, Rocha, R. D. P3 1Anthropology Department, College of Social Sciences and Philosophy, 2National Institute of Geological Sciences, College of Science, 3Department of Food Science and Nutrition, College of Home Economics, University of the Philippines, Diliman, Quezon City 1101 Philippines ------------------------------------------------- Keywords: forward and reverse reactions, equilibrium constant, Le Chatelier’s Principle…

    • 3949 Words
    • 16 Pages
    Good Essays
  • Satisfactory Essays

    chemistry experiment

    • 427 Words
    • 2 Pages

    Jul 31, 2007 - 25 posts - ‎16 authors Figure 2: Preparation of nitroguanidine from guanidine nitrate. ... 200g sulphamic acid and 124g urea were mixed in a shallow Pyrex bowl and ... [PDF] Instructions for HACH HR Nitrate Analysis www.d.umn.edu/~dlong/.../Instruction-Nitrate-HR-TNT836.pdf‎ Nitrate. Nitrate. Page 5 of 6. Summary of method. Nitrate ions in solutions containing sulfuric and phosphoric acids react with 2,6-dimethylphenol to form 4-nitro-2 ... chemical…

    • 427 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Chemistry Experiment

    • 732 Words
    • 3 Pages

    Experiment 1: TLC Analysis of Analgesic Drugs 1/23/2011 Purpose: The goal of this experiment is to test our knowledge and understanding of TLC analysis by having us do a TLC analysis of analgesics to figure out their main chemical components. Calculations: 1.) Rf = Distance spot traveled/ distance solvent traveled Results: Table 1: TLC Analysis Analgesic Drugs | Rf Value | Acetaminophen | 0.323 | Aspirin | 0.597 | Caffeine | 0.081 | Unknown 154 (Plate 1) | 0.081, 0.306, 0.597…

    • 732 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Chemistry Experiment

    • 613 Words
    • 3 Pages

    The members in our group were Whitney, myself, and Charlotte. The title of this lab was “reaction types.” The purpose of this lab was to identify the reaction type, balance equations, and observe the reactions .Our hypothesis was if there was many stations with fire, then they’re would be many combustion reactions. The procedure of the first lab was to place a solid piece of magnesium metal in the bottom of the smallest tube. Place this test tube in the test tube holder. Using tongs pick up the…

    • 613 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Organic Chemistry Experiment - Hydrocarbons Thursday, April 12, 2007 1:34 PM Purpose: To Identify an unknown Hydrocarbon Procedure: Procedure listed in handout "Organic Chemistry Experiment -- Hydrocarbons" Hazards: Open flame and hydrocarbons are flammable. Equations: 1. . 3. . 4. . 5. . Unknown #: 1B s-6 Hyd-3 (colorless liquid) Data/Observations: Test Bromine addition Alkane Mixture of hexane and dichloromethane was originally clear liquid. After adding 3 drops red color Br2/CH2Cl…

    • 413 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Chemistry Experiment 2

    • 280 Words
    • 2 Pages

    100= 10% Iron 1.9 1.9/6 * 100= 31.7% Sand 1.1 1.1/6 * 100= 18.3% Benzoic Acid 0.5 .5/6 * 100= 8.3% Salt 1.4 1.4/6 * 100= 23.4% Total 6.0 100% Questions: 1. How did your proposed procedures or flow charts at the beginning of this experiment compare to the actual procedures of this lab exercise? I had to change the process I did things a little to allow adequate time for the sand and the benzoic acid crystals to dry on the paper towels, and for the water in the paper cup to evaporate…

    • 280 Words
    • 2 Pages
    Good Essays
  • Better Essays

    Chemistry Experiment Paper

    • 2005 Words
    • 9 Pages

    of Vitamin C contained. The concentration of Vitamin C contained of these fruit juices were identified by using titration. Based on the results, the concentration of Vitamin C contained in these 3 fruit juices were obtained. The conclusion of the experiment is that different fruit juices contain different concentration of Vitamin C which is important in our daily lives. CHAPTER 1 1.0 INTRODUCTION 1.1 THEORY/LITERATURE Vitamin C is an essential nutrient in humans as it functions as a cofactor in several…

    • 2005 Words
    • 9 Pages
    Better Essays
  • Better Essays

    Exercise 1 Polymers – An introduction to Organic Chemistry Group 3 Jude Marchoni C. Tampus Meryl Marie Susan Chua Pearl Pontillas Paolo Pepito Gaia Casas I. Abstract: (Paolo) In this experiment we will be testing what would happen to the polymer when we add borax to it. A polymer is a compound made up of large molecules often in a solid state. Polymers are chemically formed by 100 to 10,000 small molecules called "monomers". Monomers occur in molecular units or patterns…

    • 850 Words
    • 4 Pages
    Better Essays
  • Satisfactory Essays

    Jamie Kim Mrs. Shaw Chemistry Honors 3 September 2014 Bubble Quality Lab Hypothesis: Bubble quality is most positively affected by the mixture of sugar and dish detergent due to the sticky consistency of sugar when emerged in liquid. Procedure: First of all, three cups were labelled according to their solution. Then, a teaspoon of dish soap and two-thirds of a cup of water was added and mixed to make cup#1. Next, a half teaspoon of table sugar was added to the same control solution and was…

    • 495 Words
    • 2 Pages
    Satisfactory Essays