Chap4 thesis experiment

Topics: Bacteria, Escherichia coli, Antibiotic resistance Pages: 5 (427 words) Published: January 4, 2014
CHAPTER 4
RESULTS AND DISCUSSION

4.1. Angelika keiskei (Ashitaba )crude non-polar extract
The crude non-polar extract from the plant Angelika keiskei (Ashitaba) was tested for the anti-bacterial property by using the Kirby – Bauer Disk Diffusion test. There were four 25uL of Ashitaba non-polar extract with different concentrations used which were 12.5 %, 25%, 50% and 100%. In line with this, respective positive controls of the two specimens and negative control were also used. The results are discussed below. 4.1.1. Test against gram positive organism

Triplicates of plates were used to identify the anti-bacterial property of Ashitaba non-polar extract against gram positive organism, Staphylococcus aureus. There were six disk namely the different concentrations of Ashitaba extract (12.5 %, 25%, 50%, 100%), the positive control ( Penicillin) and the negative control ( distilled water).

Table 4.1 Results of anti- bacterial property of Ashitaba extracts against Staphylococcus aureus sample
Zone of inhibition (mm)

12.5 %
25 %
50 %
100 %
Distilled water
Penicillin
A
0 mm
0 mm
0 mm
0 mm
0 mm
8. 0mm
B
0 mm
0 mm
0 mm
0 mm
0 mm
7.5 mm
C
0 mm
0 mm
0 mm
0 mm
0 mm
8.0 mm

Table 4.2 Interpretation of the anti- bacterial property of Ashitaba extracts against Staphylococcus aureus sample
Zone of inhibition

12.5 %
25 %
50 %
100 %
Distilled water
Penicillin
A
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant
B
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant
C
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant

Base on the table 4.1 and 4.2, the different concentrations of Ashitaba extracts (12.5 %, 25%, 50 % and 100 %) didn’t show any zone of inhibition which concludes susceptibility to the positive organism, Staphylococcus aureus. Based on http://bsac.org.uk/wp-content/uploads/2012/02/Table-10.pdf ( pa biblio na lang pls thanks )

4.1.2. Test against gram negative organism
Triplicates of plates were used to identify the anti-microbial property of Ashitaba non-polar extract against gram negative organism, Escherichia coli. There were six disk namely the different concentrations of Ashitaba extract (12.5 %, 25%, 50%, 100%), the positive control (Gentamicin) and the negative control ( distilled water).

Table 4.3 Results of anti- bacterial property of Ashitaba extracts against Escherichia coli. sample
Zone of inhibition (mm)

12.5 %
25 %
50 %
100 %
Distilled water
Gentamicin
A
0 mm
0 mm
0 mm
0 mm
0 mm
6. 0 mm
B
0 mm
0 mm
0 mm
0 mm
0 mm
6.0 mm
C
0 mm
0 mm
0 mm
0 mm
0 mm
6.0 mm

Table 4.4 Interpretation of the anti- bacterial property of Ashitaba extracts against Escherichia coli sample
Zone of inhibition

12.5 %
25 %
50 %
100 %
Distilled water
Gentamicin
A
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant
B
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant
C
Susceptible
Susceptible
Susceptible
Susceptible
Susceptible
Resistant

Base on the table 4.1 and 4.2, the different concentrations of Ashitaba extracts (12.5 %, 25%, 50 % and 100 %) didn’t show any zone of inhibition which concludes susceptibility to the negative organism, Escherichia coli.

http://www.eurl-ar.eu/data/images/ranges%20for%20qc%20strains.pdf ( pa biblio na lang pls thanks ) PA ADD na lang of more interpretation  yun na lang  thanks 
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