Cellular Respiration Lab Report
ABSTRACT
The effect of inorganic cofactor such as Magnesium to the rate of respiration of yeast was determined using Durham tube assembly with the substrate glucose. After thirty minutes, the test tube with the cofactor in the form of Magnesium sulphate MgSO4 showed the higher amount of carbon dioxide evolved which was measurable through volume and was one of the by- products of cellular respiration. This stated that the higher amount of CO2 evolved, the higher the rate of respiration. Thus, the hypothesis “If enzymes need cofactors to speed up its function, cofactors affect the rate of cellular respiration.” was accepted. Smith fermentation tube assembly containing 15 ml of 10% yeast concentration with different substrates was used to test …show more content…
Cofactors, mostly metal ions or coenzymes, are inorganic and organic chemicals that assist enzymes during the catalysis of reactions. In the performed experiment the cofactor used was Magnesium in the form of Magnesium sulfate (MgSO4). Two replicates were prepared, one with the cofactor and the one without. Since respiration produces carbon dioxide, the rate of cellular respiration could be measured through the volume of the gas evolved. Thus, the hypothesis “If enzymes need cofactors to speed up its function, cofactors affect the rate of cellular respiration.” was …show more content…
Six substrates were used. They were the following with the volume of carbon dioxide they produced in cm3. Sucrose –34.35, fructose –33.84, glucose –31.81, starch –1.57, and both lactose and distilled water got zero. Some substrates have simple compositions and are easy to break down unlike while some have complex compositions and are hard to break down during the process of respiration. With the conditions that some sugars still need to be hydrolyzed, the hypothesis “If the nature of substrates affects the cellular respiration in yeast, then the simpler the substrates the faster the cellular respiration.” was still accepted. The simpler the substrates used, the faster the rate of cellular respiration. However, this must still need to be verified and justified by further experiments that would attest stronger to it. It is recommended that another experiment dealing with the nature of the substrates under the same procedure but with different microorganisms should be
The effect of inorganic cofactor such as Magnesium to the rate of respiration of yeast was determined using Durham tube assembly with the substrate glucose. After thirty minutes, the test tube with the cofactor in the form of Magnesium sulphate MgSO4 showed the higher amount of carbon dioxide evolved which was measurable through volume and was one of the by- products of cellular respiration. This stated that the higher amount of CO2 evolved, the higher the rate of respiration. Thus, the hypothesis “If enzymes need cofactors to speed up its function, cofactors affect the rate of cellular respiration.” was accepted. Smith fermentation tube assembly containing 15 ml of 10% yeast concentration with different substrates was used to test …show more content…
Cofactors, mostly metal ions or coenzymes, are inorganic and organic chemicals that assist enzymes during the catalysis of reactions. In the performed experiment the cofactor used was Magnesium in the form of Magnesium sulfate (MgSO4). Two replicates were prepared, one with the cofactor and the one without. Since respiration produces carbon dioxide, the rate of cellular respiration could be measured through the volume of the gas evolved. Thus, the hypothesis “If enzymes need cofactors to speed up its function, cofactors affect the rate of cellular respiration.” was …show more content…
Six substrates were used. They were the following with the volume of carbon dioxide they produced in cm3. Sucrose –34.35, fructose –33.84, glucose –31.81, starch –1.57, and both lactose and distilled water got zero. Some substrates have simple compositions and are easy to break down unlike while some have complex compositions and are hard to break down during the process of respiration. With the conditions that some sugars still need to be hydrolyzed, the hypothesis “If the nature of substrates affects the cellular respiration in yeast, then the simpler the substrates the faster the cellular respiration.” was still accepted. The simpler the substrates used, the faster the rate of cellular respiration. However, this must still need to be verified and justified by further experiments that would attest stronger to it. It is recommended that another experiment dealing with the nature of the substrates under the same procedure but with different microorganisms should be