Cellular Respiration Lab Report
BIG IDEA 2
6
EDVO-Kit: AP06
Cellular Respiration
See Page 3 for storage instructions.
EXPERIMENT OBJECTIVE:
The objective of this experiment is to apply the gas laws to the function of the respirometer. Students will observe cell respiration of germinating and non-germinating seeds and describe the effects of temperature on the rate of cell respiration.
EVT AP06.120829
EX PERIMENT
AP06
Cellular Respiration
Table of Contents
Page
Experiment Components
Experiment Requirements
Background Information
3
3
4
Experiment Procedures
Experiment Overview
Taking Experimental Measurements
Experiment Results and Study Questions
Further Investigations
6
6
7
11
14
Instructor’s Guidelines
Notes to …show more content…
Photosynthesis in plants traps energy from sun by formation of covalent bonds in complex organic compounds, such as glucose. Organisms release this stored energy by the breakdown of glucose, using a set of enzymatic reactions involving many steps. Breakdown of glucose can occur in the presence of oxygen (aerobically), or in the absence of oxygen (anaerobically). Energy released by the breakdown of glucose is stored in the high energy phosphate bonds of adenosine triphosphate, ATP. Aerobic respiration yields the most energy for organisms, with every mole of glucose producing about 36 - 40 moles of ATP. Under anaerobic conditions, only 2 moles of ATP are produced.
Cellular respiration is the breakdown of organic compounds, resulting in the release of energy. The oxidative breakdown of glucose during cellular respiration produces the energy needed for life in living organisms, as given in equation 1:
1.
C6 H12 O6 + 6 O2 → 6 CO2 + 6 H2O + 686 kCal/mol.
Carbon dioxide, CO2, a by-product of cellular respiration, is required for trapping the energy of the sun by photosynthesis. Photosynthesis results in the formation of …show more content…
Students will observe cell respiration of germinating and non-germinating seeds and describe the effects of temperature on the rate of cell respiration.
LABORATORY SAFETY GUIDELINES
Experiment Procedure
1.
2.
3.
4.
5.
Wear gloves and goggles while working in the laboratory.
Exercise caution when working in the laboratory – you will be using equipment that can be dangerous if used incorrectly.
DO NOT MOUTH PIPET REAGENTS - USE PIPET PUMPS.
Always wash hands thoroughly with soap and water after working in the laboratory.
If you are unsure of something, ASK YOUR INSTRUCTOR!
LABORATORY NOTEBOOKS
Scientists document everything that happens during an experiment, including experimental conditions, thoughts and observations while conducting the experiment, and, of course, any data collected. Today, you will be documenting your experiment in a laboratory notebook or on a separate worksheet.
Before Starting the Experiment:
•
•
Carefully read the introduction and the protocol. Use this information to form a hypothesis for this experiment.
Predict the results of your experiment.
During the Experiment:
6
EDVO-Kit: AP06
Cellular Respiration
See Page 3 for storage instructions.
EXPERIMENT OBJECTIVE:
The objective of this experiment is to apply the gas laws to the function of the respirometer. Students will observe cell respiration of germinating and non-germinating seeds and describe the effects of temperature on the rate of cell respiration.
EVT AP06.120829
EX PERIMENT
AP06
Cellular Respiration
Table of Contents
Page
Experiment Components
Experiment Requirements
Background Information
3
3
4
Experiment Procedures
Experiment Overview
Taking Experimental Measurements
Experiment Results and Study Questions
Further Investigations
6
6
7
11
14
Instructor’s Guidelines
Notes to …show more content…
Photosynthesis in plants traps energy from sun by formation of covalent bonds in complex organic compounds, such as glucose. Organisms release this stored energy by the breakdown of glucose, using a set of enzymatic reactions involving many steps. Breakdown of glucose can occur in the presence of oxygen (aerobically), or in the absence of oxygen (anaerobically). Energy released by the breakdown of glucose is stored in the high energy phosphate bonds of adenosine triphosphate, ATP. Aerobic respiration yields the most energy for organisms, with every mole of glucose producing about 36 - 40 moles of ATP. Under anaerobic conditions, only 2 moles of ATP are produced.
Cellular respiration is the breakdown of organic compounds, resulting in the release of energy. The oxidative breakdown of glucose during cellular respiration produces the energy needed for life in living organisms, as given in equation 1:
1.
C6 H12 O6 + 6 O2 → 6 CO2 + 6 H2O + 686 kCal/mol.
Carbon dioxide, CO2, a by-product of cellular respiration, is required for trapping the energy of the sun by photosynthesis. Photosynthesis results in the formation of …show more content…
Students will observe cell respiration of germinating and non-germinating seeds and describe the effects of temperature on the rate of cell respiration.
LABORATORY SAFETY GUIDELINES
Experiment Procedure
1.
2.
3.
4.
5.
Wear gloves and goggles while working in the laboratory.
Exercise caution when working in the laboratory – you will be using equipment that can be dangerous if used incorrectly.
DO NOT MOUTH PIPET REAGENTS - USE PIPET PUMPS.
Always wash hands thoroughly with soap and water after working in the laboratory.
If you are unsure of something, ASK YOUR INSTRUCTOR!
LABORATORY NOTEBOOKS
Scientists document everything that happens during an experiment, including experimental conditions, thoughts and observations while conducting the experiment, and, of course, any data collected. Today, you will be documenting your experiment in a laboratory notebook or on a separate worksheet.
Before Starting the Experiment:
•
•
Carefully read the introduction and the protocol. Use this information to form a hypothesis for this experiment.
Predict the results of your experiment.
During the Experiment: