Cellular Respiration Lab
Cellular Respiration: Using the Vernier LabQuest to detect CO2 gas production in germinating peas
DESIGN
Research Question:
What is the effect of temperature on CO2 gas production in germinating peas?
Background: The process of cellular respiration, which provides energy for cell growth and development, is an important part of life in germinating seeds. It converts the chemical energy of glucose into ATP. As a germinating seed respires, CO2 is produced as a byproduct. Therefore, the amount of CO2 produced from a respiring seed can be an indicator of the amount of activity that is taking place in its cells. Germinating peas in particular undergo cellular respiration in their early stages of development, as they are often buried …show more content…
Secondly, I predict that CO2 production will increase with temperature until a certain point. Germinating peas have an optimal temperature for respiration. However, when the temperature increases significantly past optimal, it is possible that enzymes will denature, hindering or perhaps fully halting CO2 …show more content…
Warm peas:
i. One group of 25 germinating peas will be soaked in a hot water bath for 15 minutes
1. The temperature of this water will be recorded in degrees Fahrenheit in Table 1. ii. One group of 25 germinating peas will be soaked in warm tap water for 15 minutes
1. The temperature of the water will be recorded in degrees Fahrenheit in Table 1.
c. Room temperature peas:
i. One group of 25 germinating peas will be soaked in a tub filled with room temperature water for 15 minutes
1. The temperature of this water will be recorded in degrees Fahrenheit in Table 1. ii. The glass beads will be soaked in a tub filled with room temperature water for 15 minutes
1. The temperature of this water will be recorded in degrees Fahrenheit in Table 1.
4. I will locate the switch on the CO2 gas sensor to the low setting (0-10,000 parts per million [ppm])
5. Next, I will connect the gas sensor into one of the ports in the top of the Labquest
6. Using a stylus, the Labquest will be turned on. New will be selected from the file menu to start a new data collection
7. 25 room-temperature peas will be blotted dry between paper towels
8. The peas will be placed in the respiration
DESIGN
Research Question:
What is the effect of temperature on CO2 gas production in germinating peas?
Background: The process of cellular respiration, which provides energy for cell growth and development, is an important part of life in germinating seeds. It converts the chemical energy of glucose into ATP. As a germinating seed respires, CO2 is produced as a byproduct. Therefore, the amount of CO2 produced from a respiring seed can be an indicator of the amount of activity that is taking place in its cells. Germinating peas in particular undergo cellular respiration in their early stages of development, as they are often buried …show more content…
Secondly, I predict that CO2 production will increase with temperature until a certain point. Germinating peas have an optimal temperature for respiration. However, when the temperature increases significantly past optimal, it is possible that enzymes will denature, hindering or perhaps fully halting CO2 …show more content…
Warm peas:
i. One group of 25 germinating peas will be soaked in a hot water bath for 15 minutes
1. The temperature of this water will be recorded in degrees Fahrenheit in Table 1. ii. One group of 25 germinating peas will be soaked in warm tap water for 15 minutes
1. The temperature of the water will be recorded in degrees Fahrenheit in Table 1.
c. Room temperature peas:
i. One group of 25 germinating peas will be soaked in a tub filled with room temperature water for 15 minutes
1. The temperature of this water will be recorded in degrees Fahrenheit in Table 1. ii. The glass beads will be soaked in a tub filled with room temperature water for 15 minutes
1. The temperature of this water will be recorded in degrees Fahrenheit in Table 1.
4. I will locate the switch on the CO2 gas sensor to the low setting (0-10,000 parts per million [ppm])
5. Next, I will connect the gas sensor into one of the ports in the top of the Labquest
6. Using a stylus, the Labquest will be turned on. New will be selected from the file menu to start a new data collection
7. 25 room-temperature peas will be blotted dry between paper towels
8. The peas will be placed in the respiration