Observing Various Cells Under The Microscope
Biology 211 Lab section 14
New Mexico State University
Elodea Wet Mount
If cytoplasmic screening is occurring, you will see the chloroplast circulating around the cell. Cytoplasmic streaming is controlled by microfilaments in the cell’s cytoskeleton. These filaments of actin and myosin move cellular contents in an active process that uses energy released by breaking ATP down in ADP. How might a cell benefit by spending energy to circulate the cytoplasm?
Cytoplasm in a cell is responsible for cell growth, reproduction, metabolism, and eliminating waste. If the cytoplasm is circulating then the cell is able to be more efficiently functioning on its turnover rate. As a result the chloroplasts move around more, and therefore more ATP can be produced.
Leaf Epidermal Peel
How could a cell regulate the movement of carbon dioxide, oxygen, and water vapor into and out the leaf? How would the plant benefit by influencing the movement of these molecules?
The cell is able to regulate the movement of CO2, H2O, and water vapor in and out of the leaf because of the stomata. A stoma is what has the guard cells, and those are responsible for adjusting the opening and closing in the gaps between them. These are the only cells that contain chloroplasts, and can carry out photosynthesis that is “the process that converts energy from the sun into chemical energy” (Preszler et al, 2014). The plant therefore benefits from the movement of these molecules by controlling the reservation of its moisture. Tetrahymena
Can you see the ink moving into the cells? Is it enclosed within the vacuoles? What are the distributions of the ink and carmine food vacuoles in each cell? How many food vacuoles of each type are in each cell?
I did not actually see the ink “moving” into the cells, however it was extremely noticeable that once it did “move” the location of it was inside the vacuoles of the cells. This “moving” is called phagocytosis, a type of feeding behavior where “food particles are swept into the buccal cavity where they are incorporated into a membrane-bound vacuole which is then pinched off and circulated through the cell along microfilaments of the cytoskeleton” (Preszler et al, 2014). Before the ink was added, the whole cell was clear, and afterwards there were numerous red dots enclosed inside the cells AKA the vacuoles. The ink and carmine distribution of each was fairly distributed evenly. The amount of ink filled vacuoles I observed averaged about 5 per cell in the 5 minutes that we let the Tetrahymena eat.
Write an explanation of how your observations of the muscle fibers relate to your observations of processes in Elodea and Tetrahymena. What cellular structures or processes related to movement are the same in all three cells? How do these cells differ in the way movement is obtained? The muscle fibers relate to the Elodea and Tetrahymena in the way of how the ATP is responsible for the movements within the cell. In the Elodea it controls the amount of moisture. In the Tetrahymena it is attributed to moving the food along within the cell. And in the muscle tissue, the ATP contracts the muscle fibers. Pond Water
See attached drawing.
See attached drawing.
Preszler, R.W., L.L. Haas, A.L. Marion, and L.J. Urquidi. 2014. Cellular and Organismal
Biology. 10th edition. Hayden McNeil, MI.
References: Preszler, R.W., L.L. Haas, A.L. Marion, and L.J. Urquidi. 2014. Cellular and Organismal Biology. 10th edition. Hayden McNeil, MI.
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