Ccea as Biology Coursework: an Investigation to Measure the Percentage Light Transmission Through a Solution in Which Jelly Cubes Were Immersed over a 24 Hour Period
An investigation to measure the percentage light transmission, using a colorimeter, through a solution, from pH 2 – pH 9, in which jelly cubes were immersed over a 24 hour period
Interpretation
Written Communication of the Data
C1
Pepsin is an enzyme that works in the stomach and has an optimal pH between pH 1 and 4 or in acidic conditions. From our graph it can be seen that that the lowest mean percentage light transmission for pepsin is when the buffer has a pH of 2. Trypsin is an enzyme that works in the small intestine and has an optimum pH between pH 7 and 8 or in neutral conditions. From our graph it can be seen that the lowest mean percentage light transmission for trypsin is when the buffer has a pH of 8.
C2 and C3
As the pH of the pepsin buffer increases from pH 2 to pH 9 so too does the percentage light transmission through the buffer solution after a 24 hour period. Although when the trypsin buffer has a pH between pH 2 and 8 the percentage light transmission through the buffer solution after a 24 hour period decreases, but from pH 8 to pH 9 the percentage light transmission through the buffer solution after a 24 hour period increases. At a low pH (pH 2) the amount of gelatine broken down by the pepsin is high. We can tell this as this is when there is a low mean percentage light transmission (16.86%) because a lot of colour of the jelly will leak in the solution causing the colour to be deeper. But at a higher pH (pH 9) the amount of gelatine broken down by the pepsin is low. We can tell this as this as there is a low mean percentage light transmission is high (34.14%) because a little colour of the jelly will leak in the solution causing the colour to be lighter. At a low pH (pH 2) the amount of gelatine broken down by the trypsin is low so this means there is a high mean percentage light transmission (41.75%) because a little colour of the jelly will leak in the solution causing the colour to be lighter. At a high pH (pH9) the amount of gelatine
Interpretation
Written Communication of the Data
C1
Pepsin is an enzyme that works in the stomach and has an optimal pH between pH 1 and 4 or in acidic conditions. From our graph it can be seen that that the lowest mean percentage light transmission for pepsin is when the buffer has a pH of 2. Trypsin is an enzyme that works in the small intestine and has an optimum pH between pH 7 and 8 or in neutral conditions. From our graph it can be seen that the lowest mean percentage light transmission for trypsin is when the buffer has a pH of 8.
C2 and C3
As the pH of the pepsin buffer increases from pH 2 to pH 9 so too does the percentage light transmission through the buffer solution after a 24 hour period. Although when the trypsin buffer has a pH between pH 2 and 8 the percentage light transmission through the buffer solution after a 24 hour period decreases, but from pH 8 to pH 9 the percentage light transmission through the buffer solution after a 24 hour period increases. At a low pH (pH 2) the amount of gelatine broken down by the pepsin is high. We can tell this as this is when there is a low mean percentage light transmission (16.86%) because a lot of colour of the jelly will leak in the solution causing the colour to be deeper. But at a higher pH (pH 9) the amount of gelatine broken down by the pepsin is low. We can tell this as this as there is a low mean percentage light transmission is high (34.14%) because a little colour of the jelly will leak in the solution causing the colour to be lighter. At a low pH (pH 2) the amount of gelatine broken down by the trypsin is low so this means there is a high mean percentage light transmission (41.75%) because a little colour of the jelly will leak in the solution causing the colour to be lighter. At a high pH (pH9) the amount of gelatine