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catfish
Title: Factors affecting Bimodal respiration in the catfish Corydoras aeneus

Abstract: The catfish, Corydoras aeneus is an organism that respires bimodally , that it is being capable of drawing oxygen from both water and air. It carries out aerial respiration through it’s adapted posterior intestine.This experiment was done in order to determine the disadvantages of aerial respiration by manipulation of oxygen content and depth. The effect of dissolved oxygen, when the fish was placed into a cylinder of water and the oxygen concentration was determined while the number of breaths in a 10 minutes period was counted and recorded. Within the 10 minute period, three 15 seconds reps were used to observe the number of opercula beats. The results obtained were that the initial ambient level in the experiment was 5.7; the breaths taken and the operculum beats were observed and recorded. The number of operculum beats decreased, possibly due to experimental errors. The number of breaths per hour showed an increase. The oxygen content was decreased by bubbling nitrogen until about4 mg/L. This was repeated 5 times at different oxygen content levels and opercula beats and breaths recorded. At ambient level 1.23 was maintained through manipulation of depth. From the results obtained the number of operculum beats per minute increased and the number of beats per hour increased with increasing depth. The effect of depth was also determined this was done by simply filling a 1L measuring cylinder with a depth of 10cm of low oxygenated water. The fish was then placed into these conditions where the number of surface breaths/hr and opercular beats/min were recorded at various depths of 15, 20, 25 and 35cm.

Objectives:
1) To determine the effect of the dissolved oxygen concentration on the types of the respiration (aerial or aquatic) being used by the Corydoras aeneus, a bimodal respirator.
2) To determine the effect of depth on the types of respiration (aerial or



References: Biology-Online.org. “Respiration." Accessed January 30th, 2014. Education.com. "Aquatic Respiration." Accessed January 30th, 2014. http://www.education.com/science-fair/article/aquatic-respiration/ (2006). Kramer and McClure, 1980. “Aerial respiration in the catfish Corydoras aeneus”. Can. J. Zool. Accessesd Feburary 1st, 2014.http://www.lumcon.edu/bayousideclassroom/hypoxiacauses.asp . Education.com. "Fish Gills, Respiration and Ventilation Help”. Accessed February 1st, 2014.

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