Preview

Catalase Temperature Lab

Good Essays
Open Document
Open Document
740 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Catalase Temperature Lab
In this lab, I was testing different temperatures and how it affects the time catalase takes for it to break down peroxide. The problem that i was investigating is how does temperature affect the time it takes for catalase to break down peroxide. My independent variable for this lab was the temperature of the solution the enzyme is in. My dependent variable in this experiment was rate of reaction or the amount of time it takes to sink and rise. My hypothesis is that if the temperature is higher than 37℃, then it will break down the peroxide faster because yeast tends to rise faster as the temperature increases. Some constants in this lab are: the 10 mL of peroxide, the 190 mL of water, the same timer, the same sheet of filter paper and the …show more content…
When the water was at 10℃ the average rate of reaction was 1.75 mm/s. When the water was at 25℃ the average rate of reaction was 2.98 mm/s. When the water as at 37℃ the average rate of reaction was 3.45 mm/s. Finally, when the water was at 45℃ the average rate of reaction was 4.07 mm/s. Therefore, our data did support our hypothesis because we hypothesized that when the water rose in temperature the reaction rate would speed up, and so it …show more content…
The following is a description of all the variables that were controlled: the room temperature water at 25℃. This left us with with only one variable in this experiment which was the different temperatures we were testing.
If i was to design this investigation again, I would use more temperatures than just 4 to give a wider range to experiment with. Also, i would have used more than one catalase because it gives you a chance to see which catalase can work the best under each temperature. Finally, I would instead of using water and heating it up, I would use a different substance for example alcohol or juice. To verify our data, I would repeat the experiment 20

You May Also Find These Documents Helpful

  • Satisfactory Essays

    2. It is essential to use fresh and consistent batches of catalase because when the solution is left for a long period of time, it is affected by external factors such as temperature, which would impact the efficiency of the enzymes. To get the most consistent and accurate results, it is best to use the same catalase kept under the same conditions.…

    • 315 Words
    • 1 Page
    Satisfactory Essays
  • Better Essays

    Enzymes are biological catalysts. They work by lowering the activation energy needed to initiate a chemical reaction. Enzymes work within an optimal temperature and optimal pH. Enzymes are highly specific for a single substrate. The Enzyme is usually much larger in size than the substrate it binds to. In some cases, an enzyme requires something called a cofactor to begin the chemical reaction. There were four different experiments that were executed in the enzyme lab. Experiment 7.1, the first experiment, was performed to test the effect of temperature on enzymatic activity. Based on what I know about the effect of temperature on the enzymatic activity of lactase, if the lactase used in today’s lab was extracted from human cells, I hypothesize that the optimal temperature for lactase to be in is around 37 degrees Celsius, which is the average human body temperature. The second experiment performed, experiment 7.2, tested the effect of pH on enzymatic activity. Based on what I know about the effect of pH on the enzymatic activity of lactase, if the lactase used in today’s lab was extracted from human cells, I hypothesize that the optimal pH level is 7, which is the average pH level in humans. Experiment 7.3 tests enzymatic specificity. Knowing that lactase is specific for the substrate lactose, lactase will only bind to lactose and not work with the other substrate used in this experiment, maltose. The last experiment of the lab, 7.4, was done to determine the cofactors of the enzyme, lactase. I hypothesize that EDTA, a cofactor inhibitor, will have a negative effect on the reaction and lower the amount of glucose that could possibly be produced if EDTA was not present.…

    • 2988 Words
    • 12 Pages
    Better Essays
  • Good Essays

    Catalase Experiment

    • 1088 Words
    • 5 Pages

    1977). The average rate of catalase activity as seen in the chart above is 4.97 seconds while the orange is at zero seconds. During the experiment, none of the orange filtered discs rose in the given amount of time. From the data, one can see that the orange has no effect on catalase activity. Therefore the null hypothesis, the orange will not affect the catalase activity is accepted.…

    • 1088 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Controlled Variables: The first thing that will not change at the beginning of the experiment is the starting temperature of the liquids. Throughout the experiment the location of the liquids will stay the same. Also, the temperature of the room will stay the same. The mug that the liquids started in will not change so the results will have a more accurate reading.…

    • 557 Words
    • 3 Pages
    Good Essays
  • Good Essays

    The independent variable is that i want to make the temperature cold and hot. I am going to change the…

    • 402 Words
    • 2 Pages
    Good Essays
  • Powerful Essays

    Catalase Lab

    • 1797 Words
    • 8 Pages

    In this laboratory exercise we studied enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment.…

    • 1797 Words
    • 8 Pages
    Powerful Essays
  • Better Essays

    This project looks at how the temperature of an experiment can affect its reaction time. The purpose of this experiment is to determine if dissolving reactions are affected by waters temperature. I believe that if the H2O temperature increases, then the Alka-Seltzer tablet will dissolve faster because the hot water molecules will move faster colliding with the tablet particles. Water will be the independent variable due to the fact is will always stay in its same form throughout the experiment. The Manipulated variable will also be the water, since I will be changing its temperature. The Responding the variable will be the timing of how fast or slow the Alka-Seltzer tablet dissolves. And the control is the glass of water that is set at cold tap water.…

    • 946 Words
    • 4 Pages
    Better Essays
  • Good Essays

    Experiment A: 100 toothpicks per team bowl, a clock/watch with a second hand, and a pencil.…

    • 1668 Words
    • 7 Pages
    Good Essays
  • Powerful Essays

    Catalase Lab

    • 993 Words
    • 4 Pages

    Part A: As the 1 mL of fresh catalase solution was added to the 10 mL of the 1.5% H2O2 solution bubbles formed. When we transferred the 1 mL of boiled and then cooled catalase extract to 10 mL of 1.5% H2O2 it became cloudy.…

    • 993 Words
    • 4 Pages
    Powerful Essays
  • Good Essays

    Under intense heat the proteins will unravel and the lipids will melt under intense cold the membrane will freeze and fracture, therefore Betalain (stain) will leave the cell…

    • 623 Words
    • 3 Pages
    Good Essays
  • Better Essays

    The aim of this experiment was to investigate the effect of temperature on the enzyme catalase. The original research question was exploring the effect temperature would have on a yeast catalase reacting with hydrogen peroxide (H2O2). To address the latter question a series of experiments were conducted. The various temperatures experimented with were as follows: 22 degrees Celsius (room temperature), 0 degrees Celsius (freezing), 100 degrees Celsius (boiling), and 37 degrees Celsius. Along with variations in temperature, the experiment involved different amounts of the two solutions being used. Using a spot plate containing 12 wells the different mixtures of hydrogen peroxide and water were as follows: 40 drops of pure hydrogen peroxide (H2O2), 20 drops of tap water and 20 drops of hydrogen peroxide, and 10 drops of hydrogen peroxide and 30 drops of tap water. The results after all experiments combined can be summarized. The reaction between hydrogen peroxide and yeast catalase increases as the temperature increases. This experiment proved to be an effective way of meeting the aim because it left room for several readings along with room for simple changes.…

    • 1889 Words
    • 6 Pages
    Better Essays
  • Powerful Essays

    Cooper, Geoffrey M. “Enzyme Lab.” Enzyme Lab. ASM Press and Sinauer Associates, Inc. n.d. Web. 13 Nov. 2013.…

    • 1020 Words
    • 5 Pages
    Powerful Essays
  • Good Essays

    Enzyme Lab Write Up

    • 854 Words
    • 4 Pages

    A prediction that can be made for this experiment is that the higher the concentration, the faster time it takes the catalase to react with the solution. As the concentration increases from 1% to 100%, the average reaction rate of the catalase will decrease, as the catalase will speed up the reaction.…

    • 854 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Enzyme Formal Lab

    • 2542 Words
    • 11 Pages

    The purpose of this investigation is to figure out how temperature, pH, and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to occur, allowing vital processes such as digestion, ATP production, and DNA replication to occur efficiently. Using a Vernier Gas Pressure Sensor the rate of reaction of the enzyme catalase as it decomposed Hydrogen Peroxide in a test tube was measured and then results were put on the screen of a Macintosh computer using Logger Pro software and Vernier computer software. Several trials were then done with one factor, either temperature, pH, or enzyme concentration being altered, while the remaining two stayed constant. The rate of reaction of catalase should increase with enzyme concentration until a certain point until it reaches its limit. For temperature the rate of reaction of catalase should increase until the enzyme catalase denatures, and then after the rate should start to decrease. When exposed to different pH levels the rate of reaction of catalase should increase as the enzyme catalase reaches its optimal pH level, once it’s at that point the rate of reaction should then decrease as the pH level goes farther away from catalase’s optimal pH level. The data for the trials of pH and enzyme concentration support the expected hypotheses of how the reaction rate would be affected, however the data did not support the hypothesis made for the effects of temperature on an enzyme’s reaction rate due to divergent data from the expected results. (need this line or is conclusion and results of data and trends summarized with data supported hypotheses) ask if titel too long,,…

    • 2542 Words
    • 11 Pages
    Better Essays
  • Good Essays

    The Purpose of this experiment was to determine the optimal temperature and pH levels under which enzymatic reactions involving the enzyme catalase and the reactant, hydrogen peroxide, may successfully occur.…

    • 997 Words
    • 4 Pages
    Good Essays