Catalase on Hydrogen Peroxide

Powerful Essays
Abstract
The aim of this study was to test the rate of reactivity of the enzyme catalase on hydrogen peroxide while subject to different concentrations of an inhibitor. The hypothesis was that hydrogen peroxide will be broken down by catalase into hydrogen and oxygen, where a higher concentration of inhibitor will yield less oxygen, resultant of a lower rate of reaction. Crushed potato samples of equal weight were placed in hydrogen peroxide solutions of various temperatures. The results showed that less gas was produced as the concentration of the inhibitor rose. This Is because more enzymes were inhibited, and so less active sites were available for reaction.
Reasearch and rationale
Hypothesis:
Catalase will break down hydrogen peroxide in water and hydrogen. A higher concentration of inhibitor will yield a slower rate of reaction, thus yielding less oxygen in a given time.
Null hypothesis: Catalase will break down hydrogen peroxide in water and hydrogen. The concentration of the inhibitor will have no effect on the rate of this reaction. Supporting knowledge: Enzymes function as biological catalysts, increasing the rate of reactions that take place within organisms. Catalase is an enzyme found in the vast majority of organisms that are exposed to oxygen, making it very common. It catalyses the decomposition of hydrogen peroxide into water and oxygen. Hydrogen peroxide is a molecule that can attack sulphur atoms and metal ions in protein molecules, and with the aid of iron ions is potentionally mutagenic. Catalase is an extremely effective enzyme, and can break down millions of hydrogen peroxide molecules in a single second. As such, it can be seen how important and effective catalase is to oxygen-exposed organisms. As with the majority of enzymes, it has an optimum pH of approximately 7, though the optimum can vary by species. Haliotis discus discus, a type of shellfish, for example, has catalase which works at an optimum pH of 10.5. There are a

You May Also Find These Documents Helpful

  • Good Essays

    OF CATALASE ON HYDROGEN PEROXIDE The aim of the experiments is to see if increasing the surface area of the enzyme Catalase, affects the relative activity of the substrate Hydrogen peroxide. Then to observe and measure the effect the Catalase has on the chemical breakdown of the hydrogen peroxide. My theory is if you keep increasing the surface area of Catalase, the more active sites are available to join with the substrate causing an increase in the breakdown of the hydrogen peroxide producing…

    • 1225 Words
    • 5 Pages
    Good Essays
  • Better Essays

    Reaction of catalase with hydrogen peroxide AIM: I aim to find the rate of reaction between catalase and hydrogen peroxide. Enzymes such as Catalase are protein molecules that are found in living cells. They are used to speed up specific reactions in the cells. Each enzyme just performs one particular reaction so they are all very specific. Catalase enzymes found in living cells e.g. in yeast, potato or liver, speed up (in our case) the breaking down of hydrogen peroxide. The lock and key analogy……

    • 2275 Words
    • 10 Pages
    Better Essays
  • Good Essays

    Hydrogen Peroxide

    • 2041 Words
    • 9 Pages

    Hydrogen Peroxide & Inorganic Peroxy Compounds Hydrogen Peroxide Hydrogen peroxide (H2O2) is the simplest peroxide (a compound with an oxygen-oxygen single bond). It is also a strong oxidizer. Hydrogen peroxide is a clear liquid, slightly more viscous than water. In dilute solution, it appears colorless. Reactions Decomposition Hydrogen peroxide decomposes exothermically into water and oxygen gas spontaneously: 2 H2O2 → 2 H2O + O2 This process is thermodynamically favorable. It has…

    • 2041 Words
    • 9 Pages
    Good Essays
  • Good Essays

    Aim: To investigate the effect of hydrogen peroxide concentration on enzyme catalase Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also, they can speed up Metabolic reactions without being changed or used up. During a reaction, an enzyme molecule combines temporarily with the substrate. When the reaction is complete, the enzyme molecules returns to its original dorm…

    • 657 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Effects of Different Concentration of Catalyse on Hydrogen Peroxide Aim: In this investigation I will try to find how long it takes for the filter paper disc to rise up whilst varying the amounts of concentration of catalyse. Prediction: I predict that the lower the concentration of catalyse the longer it will take for the filter paper disc to rise to the surface of the tube. Equipment: 1. Hydrogen peroxide in a container 2. Flat bottom tube 3. Tweezers 4. Filter paper…

    • 571 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide. Plan Aim: To investigate the rate of the effect of Catalase on hydrogen peroxide. Introduction This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide. Enzymes are biological catalysts, which speed up the rate of reaction without being used up during the reaction, which take place in living organisms. They do this by…

    • 4612 Words
    • 19 Pages
    Good Essays
  • Powerful Essays

    Abstract The decomposition of hydrogen peroxide (H2O2) in a whole and diced wedges by with The enzyme catalase was observed. The catalase was able to break down the hydrogen peroxide In the diced banana wedge better than the whole banana because after the banana was diced that Increases the surface area allowing the breakdown to flow. The effects of temperature on enzyme In a liver sample were observed under iced, boiling, 37 degrees, and room temperature Conditions. The enzymes became completely…

    • 1787 Words
    • 8 Pages
    Powerful Essays
  • Powerful Essays

    Kinetics of Hydrogen Peroxide February 22, 2007 Chem. 1130 TA: Ms. Babcock Room 1830 Chemistry Annex PURPOSE OF THE EXPERIMENT Kinetics of Hydrogen Peroxide The major purpose of this experiment is to determine the rate law constant for the reaction of hydrogen peroxide and potassium iodide. In this experiment, the goal will be to try to measure the rate law constant at low acidity, since at low acidity, anything less than 1.0 x 10-3M, the effect of the hydrogen ion is negligible. To calculate…

    • 3056 Words
    • 13 Pages
    Powerful Essays
  • Better Essays

    Hulsey Fire Tech 107 15 November 2015 Hydrogen Peroxide According to Lee Lerner, (2008), “Hydrogen Peroxide is most widely found in homes in brown bottles containing three percent solutions (3% hydrogen peroxide and 97% water).” Lee Lerner (2008) writes “Hydrogen Peroxide is a colorless liquid that mixes with water and is widely used as a disinfectant and bleaching agent.” Hydrogen Peroxide is used in many different ways and has different hazards. “Hydrogen peroxide is a strong oxidizing agent used in…

    • 1508 Words
    • 7 Pages
    Better Essays
  • Powerful Essays

    affecting the catalytic decomposition of Hydrogen peroxide Please refer to the additional files for tables/graphs. Aim- The purpose of this investigation is to investigate how certain factors affect the catalytic decomposition of Hydrogen peroxide. I will investigate the effect of altering the mass of catalyst used on the rate of the decomposition of Hydrogen peroxide. Introduction- The decomposition of Hydrogen peroxide is a process by which Hydrogen peroxide decomposes into water and Oxygen. It…

    • 3150 Words
    • 11 Pages
    Powerful Essays