Catalase Lab Report
Title: Enzyme Catalysis of Hydrogen Peroxide by Catalase
Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue, observe the effects of changes in temperature and pH on the activity of an enzyme, perform analyses for the presence of an enzyme in tissues, and analyzing relationships between environmental conditions and enzyme activity.
Background: Cells produce proteins which are called enzymes and their job is to help reduce the amount of energy needed to start a reaction. Enzymes are catalysts which are chemical substances that reduce the amount of activation energy needed for the reaction to begin. …show more content…
After this, pour the liquid into yet another clean test tube and think about what would happen if more liver was placed in this liquid. Place the liver into the liquid mixture of hydrogen peroxide and liver and the new mixture didn’t fizz as much as with just the hydrogen peroxide and one thing of liver. The thought of what would happen when adding more liver to the mixture was correct because it was thought that the mixture would cause a smaller reaction and it did. If more hydrogen peroxide was then added to the liver from the previous steps then there would be a more gas releasing reaction than adding more liver. Test this and it was true that more hydrogen peroxide would cause a larger reaction. This proves that enzymes, such as chicken liver are reusable, while the substrates, such as hydrogen peroxide, are …show more content…
I learned that enzymes function the best when they are kept in their normal range of temperature and pH. Enzymes work the best at room temperature and at a neutral pH. Room temperature is the optimum temperature for enzymes, and I also learned that they like it better at 0°C than at 100°C. The best pH for enzymes is neutral and the second best is basic and what they do not perform well at is acidic. So I concluded that enzymes do not like to function correctly unless they are at their optimum pH levels and temperatures, which is why they need to have a fairly stable environment to function in. This tells me that the cells I worked with can only function correctly when they are in a stable environment, with a room temperature and a neutral
Problem and Objectives: How do different temperatures and different levels of pH affect the reaction rate of the enzymes in chicken liver? Demonstrate the activity of an enzyme in living tissue, observe the effects of changes in temperature and pH on the activity of an enzyme, perform analyses for the presence of an enzyme in tissues, and analyzing relationships between environmental conditions and enzyme activity.
Background: Cells produce proteins which are called enzymes and their job is to help reduce the amount of energy needed to start a reaction. Enzymes are catalysts which are chemical substances that reduce the amount of activation energy needed for the reaction to begin. …show more content…
After this, pour the liquid into yet another clean test tube and think about what would happen if more liver was placed in this liquid. Place the liver into the liquid mixture of hydrogen peroxide and liver and the new mixture didn’t fizz as much as with just the hydrogen peroxide and one thing of liver. The thought of what would happen when adding more liver to the mixture was correct because it was thought that the mixture would cause a smaller reaction and it did. If more hydrogen peroxide was then added to the liver from the previous steps then there would be a more gas releasing reaction than adding more liver. Test this and it was true that more hydrogen peroxide would cause a larger reaction. This proves that enzymes, such as chicken liver are reusable, while the substrates, such as hydrogen peroxide, are …show more content…
I learned that enzymes function the best when they are kept in their normal range of temperature and pH. Enzymes work the best at room temperature and at a neutral pH. Room temperature is the optimum temperature for enzymes, and I also learned that they like it better at 0°C than at 100°C. The best pH for enzymes is neutral and the second best is basic and what they do not perform well at is acidic. So I concluded that enzymes do not like to function correctly unless they are at their optimum pH levels and temperatures, which is why they need to have a fairly stable environment to function in. This tells me that the cells I worked with can only function correctly when they are in a stable environment, with a room temperature and a neutral