Preview

Catalase Lab

Satisfactory Essays
Open Document
Open Document
625 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Catalase Lab
Purpose
The purpose of this catalase lab is to design simple experiments to demonstrate how various factors affect the rate of enzyme activity. This lab shows how the enzyme decomposes in hydrogen peroxide.
Methods and Materials
Refer to handout attached to the back of lab
Observations
Table 1: The mL of oxygen produced with increase of catalase

30secs
60secs
90secs
120secs
150secs
180secs
Disks: 2
17ml
16ml
21ml
26ml
31ml
35ml
Disks: 4
8ml
19ml
27ml
35ml
44ml
53ml
Disks: 6
15ml
23ml
32ml
44ml
54ml
65ml
Disks: 8
25ml
42ml
70ml
90ml
100ml at 2:20 secs

Disks: 10
29ml
58ml
87ml
100ml at 1:46 secs

Table 2: The mL of oxygen produced with increase of H202

30secs
60secs
90secs
120secs
150secs
180secs
10ml
19ml
25ml
30ml
35ml
40ml
44ml
20ml
24ml
34ml
43ml
54ml
64ml
76ml
30ml
14ml
22ml
26ml
32ml
37ml
42ml
40ml
22ml
29ml
37ml
45ml
52ml
59ml
50ml
23ml
32ml
39ml
42ml
54ml
61ml

Discussion

A catalase is an enzyme that catalyzes the reduction of hydrogen peroxide. This enzyme is vital because it prevents the build-up of H202 in the body. This lab was completed to analyze and experience how H202 is decomposed in different concentrations of catalase and H202. This was done by increasing the number of catalase in the lab with the same amount of H202, and then increasing the amount of H202 with the same amount of catalase to observe results and patterns.
Graph 1 shows the increased decomposition of H202 that was produced when the amount of catalase was increased by 2 disks every time. The more catalase that was added, the faster the decomposition was. The fastest and most efficient decomposition was when there were 10 catalase filter papers in 10mL of H202. The graduated cylinder was filled with oxygen in 1 minute and 46 seconds.
Graph 2 shows that the decomposition rate stayed relatively similar with two catalase disks and different, increasing amounts of H202. This
Continue Reading
View Writing Issues

You May Also Find These Documents Helpful

  • Good Essays

    Bio Lab Essay K101
    • 1732 Words
    • 7 Pages

    Bio Lab Essay K101

    The main objective of this lab was to observe the activity of enzyme peroxidase in real time under different experimental conditions. To see how peroxidase reacts with its substrate guaiacol under different conditions. We measure the amount of substances per test tube and then combine all of them together. Each test tube contains a measured amount of any listed substances including H₂O. Each measured amount of peroxidase along with its substrate guaiacol, and other listed substances will show how actively the enzyme oxidizes the substrate. To measure the…

    • 1732 Words
    • 7 Pages
    Good Essays
    Read More
  • Better Essays

    AP Biology enzyme cataylst lab report
    • 2040 Words
    • 9 Pages

    AP Biology enzyme cataylst lab report

    Purpose/Problem: There are four parts to the Enzyme Catalyst lab - Activity A, B, C, and D. In activity A, the characteristics of enzyme actions will be observed. The main purposes are to determine the rate of an enzyme catalyzed reaction, to study the characteristics of an enzyme mediated reaction, and to observe the effect of heat on enzyme activity. The purpose of activity B is to use the Titration Protocol to determine the initial amount of H2O2 present in a solution. The amount will be the baseline for activities C and D. The purpose of activity C is to determine the rate at which H2O2 spontaneously decomposes when exposed to room temperatures and ambient light for 24 hours. The purpose of activity D is to determine the rate at which catalase decomposes H2O2. After adding H2SO4 for different time lashes, etc., the resulting data will be graphed at which the catalase decomposed by catalase.…

    • 2040 Words
    • 9 Pages
    Better Essays
    Read More
  • Good Essays

    Formal Lab Report 2 Final
    • 1572 Words
    • 7 Pages

    Formal Lab Report 2 Final

    Purpose: Cells produce toxic wastes, in this experiment hydrogen peroxide, and without some sort of molecule to break it down the cell will die, along with the organism itself. However with the aid of an enzyme, catalase, hydrogen peroxide is able to be broken down into an intermediate and thereafter harmless substances water and oxygen. The goal of this lab is to measure the reaction rate of this process in different substances such as a liver, a vegetable, and breast tissue. By using variables such a pH and temperature we are able to how the rate of reaction is altered or improved. If it has improved, the optimum has been discovered and the enzyme will create a higher reaction rate. If above the optimal points, proteins will denature and the reaction rate will remain the same. This is vital for cellular activity for if homeostasis is not reached enzymatic activity will decrease or the enzymes will simply denature and the toxicity within the cell will increase killing the cell.…

    • 1572 Words
    • 7 Pages
    Good Essays
    Read More
  • Good Essays

    AP Bio Lab Report
    • 814 Words
    • 8 Pages

    AP Bio Lab Report

    the catalase was the enzyme. The hydrogen peroxide formed a decomposition reaction as it was broken…

    • 814 Words
    • 8 Pages
    Good Essays
    Read More
  • Satisfactory Essays

    Catechol Oxidase Lab Report
    • 243 Words
    • 1 Page

    Catechol Oxidase Lab Report

    The enzyme in this experiment was catechol oxidase, extracted from a potato. In the experiment, when substrate concentration was held constant, and the different enzyme serial dilutions were being tested, the reaction rate was expected reaction rate was expected to increase until all enzymes are saturated, then the reaction rate would level off. First, dilute the enzyme and substrate. After,…

    • 243 Words
    • 1 Page
    Satisfactory Essays
    Read More
  • Satisfactory Essays

    Lab 1 again
    • 768 Words
    • 3 Pages

    Lab 1 again

    Figure 1: The reaction rates of enzyme-catalysed reactions of hydrogen peroxide into diluted water depending of the varying percentage concentrations of catalase. Mean rates (±SE) of 5 separate experiments (n=5) are shown. Line of best fit (y = 0.0002x + 0.0038 R² = 0.9154) is presented using the trend line function in Excel.…

    • 768 Words
    • 3 Pages
    Satisfactory Essays
    Read More
  • Good Essays

    Catechol Oxidase Lab
    • 923 Words
    • 4 Pages

    Catechol Oxidase Lab

    Have you ever wondered why the inside of your apple turns brown if you leave it on the counter after taking a bite? This is because there is a compound within the apple called catechol which, when exposed to the air, becomes oxidized by the oxygen in the air and produces benzoquinone, which makes the apple appear brown (Andrew Clapper’s Online Journal, 2007). This chemical reaction is catalyzed by the enzyme catechol oxidase (Figure A.1). Enzymes are proteins in cells that speed up chemical reactions (Enzyme Procedure Guide, 2013). The catechol oxidase lowers the amount of energy needed in order for the catechol to be oxidized, which speeds the process up.…

    • 923 Words
    • 4 Pages
    Good Essays
    Read More
  • Powerful Essays

    Catalase Lab
    • 1486 Words
    • 6 Pages

    Catalase Lab

    The purpose of this lab was to test if ethanol affects the reaction involving hydrogen peroxide and catalase. Tests were performed by putting chicken liver, ethanol solution (diluted ethanol solution for other trials) and hydrogen peroxide in a test tube with a side arm, and having a rubber tube lead the oxygen gas into a gas collection tube. Results from the tests showed a negative correlation, this means that the more diluted the solution of 95% ethanol was, the less oxygen gas collected. Controlling a number of factors which include human error, temperature change, pH levels, substrate concentrations and ensuring a controlled experimental environment will work to increase the accuracy of the experiment.…

    • 1486 Words
    • 6 Pages
    Powerful Essays
    Read More
  • Good Essays

    Lab 6 enzymes
    • 1000 Words
    • 5 Pages

    Lab 6 enzymes

    Preparation Step 1: Research (online) the reaction between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by measuring the height of the bubbles produced.…

    • 1000 Words
    • 5 Pages
    Good Essays
    Read More
  • Powerful Essays

    Catalase Lab
    • 1797 Words
    • 8 Pages

    Catalase Lab

    Each lab was assigned to a group A, B, C and D in our class performed this experiment. The data presented in this report will reflect the average rates of change in O2 ml/min of our class results. In the later discussion sections, it will become…

    • 1797 Words
    • 8 Pages
    Powerful Essays
    Read More
  • Good Essays

    Catalase Lab Report
    • 370 Words
    • 2 Pages

    Catalase Lab Report

    Purpose: To observe the effects of hydrogen peroxide on living tissue and non-living materials, showing the presence or absence of catalase as observed by the presences of bubbles.…

    • 370 Words
    • 2 Pages
    Good Essays
    Read More
  • Good Essays

    How Does Temperature Affect The Rate Of An Enzyme Reaction
    • 443 Words
    • 2 Pages

    How Does Temperature Affect The Rate Of An Enzyme Reaction

    Introduction “The Enzyme Reaction” An enzyme is a protein that acts as a catalyst, which brings out a biochemical reaction. A Catalase enzyme, the enzyme tested in this experiment, is found in almost all living organisms that are exposed daily to oxygen (such as fruits, vegetables and animals). Background Information The Catalase enzyme in this experiment is known for being less affective the warmer the temperature is. According to “Science fair projects” an enzyme becomes unstable at higher temperatures and the shape of the enzyme changes. The enzyme is also used to remove hydrogen peroxide from clothing in the textile industry.…

    • 443 Words
    • 2 Pages
    Good Essays
    Read More
  • Satisfactory Essays

    Hydrogen Peroxide Experiment Lab Report
    • 356 Words
    • 2 Pages

    Hydrogen Peroxide Experiment Lab Report

    Aim: To see find out how the amount of catalase correlates with the amount of gas created when in combination with hydrogen peroxide.…

    • 356 Words
    • 2 Pages
    Satisfactory Essays
    Read More
  • Powerful Essays

    Ib Bio Experiment on Effect of Substrate Conc on Enzyme Activity
    • 1211 Words
    • 5 Pages

    Ib Bio Experiment on Effect of Substrate Conc on Enzyme Activity

    To investigate the effect of substrate concentration (manipulated by increasing concentration of hydrogen peroxide) on the rate of enzyme activity of catalase, produced by liver cells, on the decomposition of hydrogen peroxide.…

    • 1211 Words
    • 5 Pages
    Powerful Essays
    Read More
  • Powerful Essays

    The Effect Of Ph On Catalase Activity
    • 1528 Words
    • 7 Pages

    The Effect Of Ph On Catalase Activity

    We then poured 30 ml of the catalase solution into three 50 ml beakers. We also added 10 ml of water to each of the 50 ml beakers containing the catalase solution. For the catalase activity measuring apparatus kit, we filled the container halfway with water, and we filled the graduated cylinder with water all the way to the 0 ml mark. We also made sure that the hose was under the graduated cylinder in the container of water. Next, the group poured in 40 ml of 1% Hydrogen Peroxide and catalase solution into the gas stopper. Once the reaction started every 5 seconds, we would record how much oxygen was produced for 30 seconds. We repeated that for two trials. We then used a plastic cup to lower the pH of the catalase solution from 6 to 3.5, by adding 1 M of Hydrochloric acid to the solution. To make sure it was a pH that was needed, we used a pH test strip. We added 40 ml of 1% Hydrogen Peroxide and the altered catalase solution into the gas stopper. Once the reaction started every 5 seconds, we would record how much oxygen was produced for 30 seconds. We repeated that process for two trials For the third experimental group we altered the pH of the catalase solution from a pH of 6 to a pH of 8 by adding 1 M of Sodium Hydroxide to the solution. To make sure it was a pH that was needed, we used a pH test strip. We added 40 ml of 1% Hydrogen Peroxide and altered catalase…

    • 1528 Words
    • 7 Pages
    Powerful Essays
    Read More