Preview

Carbohydrates Report

Better Essays
Open Document
Open Document
1471 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Carbohydrates Report
Viviana Veber Bio 1510 Sec 21 Carbohydrates

Introduction:
The objective of this experiment was to identify the reaction of carbohydrates in Benedict’s test, Barfoed’s test and Iodine test. Carbohydrates are essential to living organisms, and the principal role of carbohydrates is the production of energy. Carbohydrates are groups of sugars that contain carbon, oxygen, and hydrogen in a 1:2:1 ratio. Three main units of carbohydrates are monosaccharides, disaccharides, and polysaccharides. Monosaccharides are a single chain of CH2O while disaccharides are two units bonded together through dehydration reaction but they are both considered sugars. Polysaccharides on the other hand are a combination of three or more units of monosaccharides put together. Carbohydrates are formed by the combination of carbon dioxide and water molecules. The carbohydrates contain two specific functional groups in which are the hydroxyl groups and carbonyl groups. In the carbohydrates, if the sugar contains aldehyde group it called reducing sugar and called non-reducing sugar if didn’t have the aldehyde group. The Benedict’s test shows us which sugars are reducing or non-reducing. Benedict's reagent, which contains Cu2+ ions in alkaline solution with sodium citrate added to keep the cupric ions in solution. The alkaline conditions of this test causes isomeric transformation of ketoses to aldoses, resulting in all monosaccharides and most disaccharides reducing the blue Cu2+ ion to cuprous oxide (Cu2O), a brick red-orange precipitate. Examples of reducing sugar are glucose. Examples of non-reducing sugar are sucrose.
Monosaccharide, which is smallest carbohydrates molecules, can be described by the number of carbons in the chain so that a monosaccharide with five carbons in a chain is a pentose and one with six carbons is a hexose. A monsaccharide contains one sugar unit, and has three to seven carbon atoms. Monosaccharides have a hydroxyl group bonded to each carbon

You May Also Find These Documents Helpful

  • Good Essays

    Molisch Test Lab Report

    • 419 Words
    • 2 Pages

    (Food Tests - Benedict's Test for Reducing Sugars) Reducing sugars contain an aldehyde functional group or ketone group in open chain form which can be converted into aldehyde easily. Both Fehling’s test and Benedict’s test are used to determine and identify the presences of reducing sugars in a solution. All monosaccharides are reducing sugars as well as some easily hydrolyzed disaccharides as they possess the free aldehyde or ketone group to be…

    • 419 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Monosaccharides are classified according to the number of carbon atoms, if it contains 3 carbons they are called Trioses e.g. glyceraldehydes which plays a part in respiration and photosynthesis. Pentoses e.g. ribose, contain 5 carbons and are used in the synthesis of nucleic acids (RNA and DNA), providing a strong sugar-phosphate backbone.…

    • 757 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Introduction: By completing lab exercise 6 : Biologically Important Molecules, we are able to perform tests such as the Benedicts Test for Reducing Sugars, Iodine Test, Biuret Test, Sudan IV Test, and lastly the Grease-Spot Test that detect the presence of biologically important carbohydrates, protein lipids and nucleic acids. Most known compounds in living organism are if fact carbohydrates, lipids, proteins, and nucleic acids. Each of these macromolecules are tested differently. The Benedicts test identifies reducing sugars based on their ability to reduce cupric ions to cuprous oxide at basic pH resulting in a green to reddish orange color. The basis for the iodine test for starch is since starch is a coiled polymer of glucose, the iodine interacts with…

    • 1210 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Analysis: Lab 1 Identifying Carbohydrates There were many ranges of color changes in the Benedicts test and Iodine stain. For the Benedicts test, this oxidation/reduction response changes the arrangement of the reducing sugar creating a colored precipitate. The change in color is correlated to the first concentration of the reducing sugar. For the iodine test, the three dimensional structure of various polysaccharides permits them to react with the iodine stain to produce a certain color. As far as the molecular level, there is an OIL – RIG method. Oxidation occurs when a substance gains oxygen or loses electrons or hydrogen (OIL – oxidation is loss) and reduction occurs when a substance loses oxygen or gains electrons or hydrogen (RIG – reduction is gain). Also, the distilled water (test tube one) served as a control for the experiment. Since polysaccharides are complex, the 3- dimensional sugars are often known as “complex carbs”. These molecules are composed of many elements of glucose linked together by the dehydration synthesis.…

    • 1028 Words
    • 5 Pages
    Good Essays
  • Good Essays

    In doing the lab, one was able to determine the characteristics of the given solutions, containing different macromolecules, whilst doing the multiple tests. The tests performed were, iodine testing for starch and glycogen, Benedict’s test for reducing sugars, and the Biuret’s test for protein. Depending on the test performed, certain results acquired, clearly identified the macromolecules present in each solution. With the aid of the results from the lab, the unknown solution will also be determined.…

    • 826 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    Ap Biology Unit 1 Summary

    • 2660 Words
    • 11 Pages

    * Monosaccharides can be distinguished by the carbonyl group they possess- aldehyde or ketone- and the number of atoms in their carbon backbone…

    • 2660 Words
    • 11 Pages
    Powerful Essays
  • Powerful Essays

    The control samples have a known outcome; in this lab both positive and negative controls were used to determine the identity of the unknown solution. The macromolecules being tested in this lab were carbohydrates (monosaccharides and polysaccharides) and proteins. To identify the presence of these macromolecules in a substance, three different tests were performed. Lugol 's iodine solution was used for identification of starch and glycogen - polysaccharides - in the twelve solutions. A positive outcome of the test results in a colour change; blue-black in the presence of starch and a red-brown in the presence of glycogen. A negative outcome results in no colour change and all solutions remain a very pale yellow (Pavia, 2005). Benedict 's solution was used to identify the presence of reducing sugars; the aldehyde functional group is the part that reacts in the test. A positive indicator of the test is the formation of a coloured precipitate of the blue solution, ranging from yellow-green to red-brown (Hequet and Abidi, 2006).…

    • 1307 Words
    • 11 Pages
    Powerful Essays
  • Good Essays

    Discussion: By observing the table above, we could see that Benedict's test was for reducing sugars, iodine test was for the presence of starch, filter paper was for the presence of fatty acids, and the Biuret test was for amino groups present in proteins. Benedict's solution was used to test for the presence of simple sugars, such as glucose (monosaccharide). When heated, the solution mixed with monosaccharides produced a reddish-orange colour. This was because Benedict's solution is composed of sodium citrate, sodium carbonate, and cupric sulfate pentahydrate. When solution is heated, an oxidation-reduction reaction occurs: cupric ion (Cu+2) oxidizes into a cuprous ion (C+) and precipitates into cuprous oxide (Cu2O) because Benedict's solution loses an oxygen (Cu+2…

    • 600 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Carbohydrates, sugar molecules, are known as “fast fuel” in the body, and they contain base elements of carbon, oxygen and hydrogen. (Lea, 2002) The simplest carbohydrate molecule is known as a monosaccharide, which is a simple sugar. Monosaccharide’s can be joined together by a covalent glycosidic bond to create more complex sugars such as disaccharides (2 monosaccharide’s), oligosaccharides (between three to ten monosaccharide’s) and polysaccharides (consists of several monosaccharide’s). (Karp, 2010) Starch is the most common nutritional polysaccharide, which consists of two polymers: amylose and amylopectin. Amylose is unbranched whereas amylopectin is branched. Structural polysaccharides consist of cellulose, which is a major component of plant cell walls; chitin, which is a tough outer covering of invertebrates; and glycosaminoglycans (GAGs). (Karp, 2010)…

    • 2144 Words
    • 9 Pages
    Better Essays
  • Satisfactory Essays

    Carbohydrates-Carbohydrates is a natural compound like sugars, starches, celluloses, and gums. Carbohydrates have six main functions in the body. They provide energy and regulate blood glucose, break down fatty acids, flavor and sweeteners, dietary fiber and they spare the use of proteins for the use of energy. Carbohydrates are broken down into two groups complex and simple carbohydrates There are simple carbohydrates such as glucose, lactose, and fructose, and you find these in cakes, candy bars, fudge, cereal, and milk. The simple carbohydrates are broken down into two types the monosaccharides and the disaccharides. The monosaccharides are made up of one sugar and are broken down quickly by the body and are the building blocks for complex carbs. The disaccharides consist of two monosaccharides that are linked together. Complex Carbohydrates are also broken down into two types the oligosaccharides and the polysaccharides. The oligosaccharides are complex and consist of 3 to 10 sugars. They are rich in fiber and they take longer to digest than simple carbohydrates. The polysaccharides have a great amount of vitamins, minerals, and fiber and contain even more sugar than the oligosaccharides. Some examples of complex carbohydrates are cellobiose, isomaltose, rutinose, and trehalose and you find some of these in asparagus, broccoli, spinach, and wild…

    • 362 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Carbs Research Paper

    • 406 Words
    • 2 Pages

    Carbs are our body’s primary energy source, therefore they are a crucial part of a healthy diet. Carbs should not be avoided all together, that being said there are “good carbs” and “bad carbs”. Simple carbs have been labeled as bad carbs, while complex carbs have taken on the name of good carbs, and this is based on what our bodies do with them. Complex carbs such as whole grains and legumes take more time for the body to break down and use so they give us a more even or extended amount of energy. Simple carbs get their name because they are easy to digest and therefore are of little value to your body. They are also high in sugar and low in fiber. This is where I get confused because fruits and veggies are simple carbs composed of basic sugars but they are good for you unlike cake and cookies. The reason for this is because they are also full of fiber so this changes how our bodies…

    • 406 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Carbohydrate Diet Analysis

    • 1060 Words
    • 5 Pages

    After many years of thinking I was a good eater, I realized there are many adjustments I need to make to my regular diet for it to be more nutritious. By reviewing my regular eating patterns, I realized I eat too many carbohydrates a day. The amount of carbohydrates I consumed was more than double the RDA, Recommended Dietary Allowance. To help reduce my carbohydrate consumption, I plan on eating less cereal because that has become a go-to meal for me at college. Cereal can add up in sugar and carbohydrates quickly when it is eaten as a meal or in large amounts. I also plan to eliminate the amount of bread I eat, by reducing the amount of sandwiches I eat or resorting to salads instead. I will also try to eat less breaded meat and pick grilled…

    • 1060 Words
    • 5 Pages
    Good Essays
  • Good Essays

    iii. Number of carbons also varies in monosaccharides 3. Trioses (3), pentoses (5), hexose (6)…

    • 712 Words
    • 3 Pages
    Good Essays
  • Good Essays

    etched

    • 686 Words
    • 3 Pages

    A: There are twenty amino acids, with that there are only three types of monosaccharides; the large variety is because of the constant change in the R groups. The three monosaccharides are fructose, galactose and glucose. In comparison, there are only two types of fatty acids, those being, unsaturated and saturated. The group that exhibits the greatest structural variability is amino acids…

    • 686 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Lab 1 Carbohydrates

    • 2254 Words
    • 21 Pages

    In the remaining two experimental procedures, colorimetric assays will be used to detect the presence of certain carbohydrates. Glucose oxidase and Iodine Reactions will be…

    • 2254 Words
    • 21 Pages
    Good Essays