Buffer Lab

[pic] Drops |Water(HcL) |Water(NaOH) |Liver(HcL) |Liver(NaOH) |Egg White(HcL) |Egg White(NaOH0) |Potato(HCl) |Potato(NaOH) |Buffer(HCl) |Buffer(NaOH) | |0 |7 |4 |7.4 |5 |8.2 |7 |6.9 |4 |10.7 |10 | |5 |4.5 |7 |6.9 |6 |7.5 |8 |6.2 |5 |10.5 |10 | |10 |2.7 |9 |6.3 |6 |7 |9 |5.7 |5 |10.4 |11 | |15 |2.6 |12 |5.8 |6 |6.4 |9 |5.3 |6 |10.3 |12 | |20 |2.5 |12 |5.4 |6 |4.5 |10 |4.9 |7 |10.2 |12 | |25 |2.4 |13 |5.1 |6 |3.5 |10 |4.6 |8 |10.1 |13 | |30 |2.3 |13 |4.8 |6 |3.3 |11 |4.2 |8 |10 |13 | |

1. The effects on water from hcl is that the ph went 1000x weaker than it went 100x weaker than it just didn’t change anymore. The effects on water from the NaOH are that it started with a 4 pH than went up to a 7 pH and it started to just keep the same pH.

2. The average pH change for the 30 drops of HCl added to the biological material is 4.3.

3. The average pH change for the 30 drops of NaOH added to the biological material is 8.3.

4. (Q2/Q3) compare to the changes in tap water with both the acid and base because its like putting 30 drops in water the first time when you actually put in just 5 drops. The pH changes drastically.

5. The biological materials respond to changes in pH by getting weaker and weaker at a steady pace.

6. The buffer solution barely responds to the HcL, it only moves 0.1 to 0.2 each 5 drops. On the other hand the buffer solution responds to the NaOH by changing pH each 10 drops.

7. The pH response of the buffer solution is more like that of distilled water even though the water pH changes drastically the first 5 drops it goes at a very low pace from 0.1 to 0.2.

8. The reaction of the buffer solution serve as a model because it the pH doesn’t change that much. It looks like it absorbs it.

9. Buffers would aid the maintenance of homeostasis within a living cell in a changing environment.