Boerhavia Mellita Lab Report

Collection of Plant Material and Extraction The experimental plant Boerhavia diffusa was collected from the areas in and around Coimbatore and duly authenticated from Botanical Survey of India, TNAU, Coimbatore. The authentication number is BSI/SRC/5/23/2013-14/Tech/1041. Fresh leaves of Boerhavia diffusa was extracted by Soxhlet apparatus for 72 hours using 95% ethanol. The extract obtained is filtered and the solvent was removed by using rotary evaporator apparatus and used for the study.
Chemicals
Total cholesterol, triglycerides, HDL-C diagnostic kits, streptozotocin, isoproterenol, were obtained from Sigma, Chemico Co, USA. All the other chemicals and solvents used were of analytical grade.

Experimental Animals The animals were treated
After 3 days diabetes was confirmed by the presence of high blood glucose levels.
Induction of myocardial infarction (MI) in experimental animal
Myocardial infarction was induced in the rats (Group 2, Group 3 and Group 4) by intraperitoneal injection of isoproterenol hydrochloride at a dose of 80 mg/kg body weight for two consecutive days (after pre-treatment with leaf extract and drugs (glibenclamide and statin) for a period of 45 days).
Experimental Design Overnight fasted diabetic male Wistar rats with blood glucose level above 200 mg/dL were used for the study. They were divided into four groups of six animals each

Groups Treatment
T1 Untreated control which received standard pellet diet and water throughout the experimental period.
To this, 1.0 ml of 5N H2SO4 was added and digested in a digestion rack till it became light brown. It was then cooled to room temperature; one or two drops of 2N HNO3 were added, digested again till it became colourless. The Kjeldhal flask was cooled and 1.0 ml of water was added and heated in a boiling water bath for 5 minutes. 1.0 ml of 2.5 per cent ammonium molybdate was then added followed by 0.1 ml of ANSA. The volume was made upto 10 ml with distilled water. The absorbance was measured at 660 nm within 10