Blood Glucose Regulation Lab
LABORATORY REPORT
Activity:
Blood Glucose Regulation
Name:
Jazmine Ricine Parham
Instructor:
Professor Deno
Date:
12.03.2014
Predictions
Plasma glucose levels will be highest immediately after the meal (0 hr)
Plasma ketone levels will be highest
1-3 hours after the meal
Plasma insulin levels will be highest immediately after the meal (0 hr)
Plasma glucagon levels will be highest before the meal (fasting)
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
These variables were controlled because any change in them would have greatly effected the DV and IV and the whole outcome of the experiment. 5. What type of assay was used to measure plasma glucose and ketone levels?
Enzymatic analysis was used to measure plasma glucose and ketone levels.
6. What type of assay was used to measure plasma insulin and glucagon levels?
Radioimmunoassay was used to measure plasma and insulin and glucagon levels.
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.4
6.4
6.3
5.6
4.8
Glucose (mmol/l)
Sub. 2
Sub. 3
5.3
5.4
6.3
6.4
6.3
5.5
4.8
6.3
5.7
4.6
average
5.4
6.4
Sub. 1
109
106
6.3
5.6
4.7
68
58
68
Ketones (µmol/l)
Sub. 2
Sub. 3
115
95
109
99
Laboratory Report/ Jazmine Ricine Parham/ Blood Glucose Regulation/ Professor Deno/ 12.03.2014/ Page [1] of [4]
53
64
70
71
53
47
average
106
105
64
58
62
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
Plasma glucose concentration
Activity:
Blood Glucose Regulation
Name:
Jazmine Ricine Parham
Instructor:
Professor Deno
Date:
12.03.2014
Predictions
Plasma glucose levels will be highest immediately after the meal (0 hr)
Plasma ketone levels will be highest
1-3 hours after the meal
Plasma insulin levels will be highest immediately after the meal (0 hr)
Plasma glucagon levels will be highest before the meal (fasting)
Materials and Methods
Dependent Variable plasma levels of glucose, ketones, insulin, and glucagon
Independent Variable food and beverage intake
Controlled Variables physical activity, caffeine and alcohol intake, gender, age, BMI
4. Why were physical activity and caffeine and alcohol intake the controlled variables?
These variables were controlled because any change in them would have greatly effected the DV and IV and the whole outcome of the experiment. 5. What type of assay was used to measure plasma glucose and ketone levels?
Enzymatic analysis was used to measure plasma glucose and ketone levels.
6. What type of assay was used to measure plasma insulin and glucagon levels?
Radioimmunoassay was used to measure plasma and insulin and glucagon levels.
Results
Table 2: Glucose and Ketone Concentration in Blood Plasma
Fasting
Immediately post meal
I hr post meal
2 hr post meal
3 hr post meal
Sub. 1
5.4
6.4
6.3
5.6
4.8
Glucose (mmol/l)
Sub. 2
Sub. 3
5.3
5.4
6.3
6.4
6.3
5.5
4.8
6.3
5.7
4.6
average
5.4
6.4
Sub. 1
109
106
6.3
5.6
4.7
68
58
68
Ketones (µmol/l)
Sub. 2
Sub. 3
115
95
109
99
Laboratory Report/ Jazmine Ricine Parham/ Blood Glucose Regulation/ Professor Deno/ 12.03.2014/ Page [1] of [4]
53
64
70
71
53
47
average
106
105
64
58
62
Glucose Concentration in Blood Plasma
Ketone Concentration in Blood Plasma
1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.
Plasma glucose concentration