Biosynthesis Of Ethanol From Sucrose Fermentation

Topics: Ethanol, Distillation, Azeotrope Pages: 4 (444 words) Published: April 13, 2015
Karmini Mondal
Chemistry 245- 061

Biosynthesis of Ethanol from Sucrose

Introduction:
Ethanol can be created using two methods: acid catalysed hydration of ethylene and through fermentation of sucrose. The purpose of this experiment was to use fermentation to produce ethanol from commercial sucrose (store-bought sugar), using fractional distillation of the fermented solution. The percent ethanol in the fermented solution was then calculated. Mechanism:

Results:
Mass of conical vial
10.36 g
Mass of conical vial with distillate
11.17 g
Mass of distillate (mass of vial-mass of vial with distillate) 11.17 g – 10.36 g = 0.81 g
Volume of distillate using automatic pipet
1 mL
Density of distillate (mass of distillate/volume of distillate)

Percent composition by weight of ethanol using table on page 006 ~90%
Percent composition by volume of ethanol using table on page 006 ~93.3%

Percent Yield:
Theoretical Yield:
1 sucrose→4 ethanol

100 % weight and volume percent EtOH
Experimental Yield:
(from table): 93.3% EtOH by volume.
Percent Yield:

Discussion:
The percent yield of ethanol from sucrose was successfully high, at 93.3 %. The final mass of the distillate obtained after fermentation and the fractional distillation process was 0.81g. The distillate was collected when the temperature reached approximately 50-70°C. The amount of ethanol that should have been produced was 13.66 ml, had the entire distillate (over 100 ml) been distilled fractionally . The density of the distillate obtained was 0.81 g/mL; therefore, the percent ethanol by weight was approximately 90%. The distillate contained mostly ethanol, but also contained water because due to the azeotropic mixture water and ethanol mix to produce, consisting of 95% ethanol and 5% water by weight. This experiment was a success due to the high percent yield of ethanol from the fermentation of sugar.

Errors introduced to this lab consisted of using unclean glassware...
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