Bioreactor Design for Tissue Engineering
JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 100, No. 3, 235–245. 2005 DOI: 10.1263/jbb.100.235
© 2005, The Society for Biotechnology, Japan
REVIEW
Bioreactor Design for Tissue Engineering
Ralf Pörtner,1* Stephanie Nagel-Heyer,1 Christiane Goepfert,1 Peter Adamietz,2 and Norbert M. Meenen3
Technische Universität Hamburg-Harburg, Bioprozess- und Bioverfahrenstechnik, Denickestr. 15, 21071 Hamburg, Germany,1 Universitätsklinikum Eppendorf, Institut für Biochemie und Molekularbiologie II, Martinistr. 52, 20246 Hamburg, Germany,2 and Universitätsklinikum Eppendorf, Unfall-, Hand- und Wiederherstellungschirurgie, Martinistr. 52, 20246 Hamburg, Germany3
Received 7 March 2005/Accepted 31 May 2005
Bioreactor systems play an important role in tissue engineering, as they enable reproducible and controlled changes in specific environmental factors. They can provide technical means to perform controlled studies aimed at understanding specific biological, chemical or physical effects. Furthermore, bioreactors allow for a safe and reproducible production of tissue constructs. For later clinical applications, the bioreactor system should be an advantageous method in terms of low contamination risk, ease of handling and scalability. To date the goals and expectations of bioreactor development have been fulfilled only to some extent, as bioreactor design in tissue engineering is very complex and still at an early stage of development. In this review we summarize important aspects for bioreactor design and provide an overview on existing concepts. The generation of three dimensional cartilage-carrier constructs is described to demonstrate how the properties of engineered tissues can be improved significantly by combining biological and engineering knowledge. In the future, a very intimate collaboration between engineers and biologists will lead to an increased fundamental understanding of complex issues that can have an impact on tissue formation in bioreactors.
[Key
© 2005, The Society for Biotechnology, Japan
REVIEW
Bioreactor Design for Tissue Engineering
Ralf Pörtner,1* Stephanie Nagel-Heyer,1 Christiane Goepfert,1 Peter Adamietz,2 and Norbert M. Meenen3
Technische Universität Hamburg-Harburg, Bioprozess- und Bioverfahrenstechnik, Denickestr. 15, 21071 Hamburg, Germany,1 Universitätsklinikum Eppendorf, Institut für Biochemie und Molekularbiologie II, Martinistr. 52, 20246 Hamburg, Germany,2 and Universitätsklinikum Eppendorf, Unfall-, Hand- und Wiederherstellungschirurgie, Martinistr. 52, 20246 Hamburg, Germany3
Received 7 March 2005/Accepted 31 May 2005
Bioreactor systems play an important role in tissue engineering, as they enable reproducible and controlled changes in specific environmental factors. They can provide technical means to perform controlled studies aimed at understanding specific biological, chemical or physical effects. Furthermore, bioreactors allow for a safe and reproducible production of tissue constructs. For later clinical applications, the bioreactor system should be an advantageous method in terms of low contamination risk, ease of handling and scalability. To date the goals and expectations of bioreactor development have been fulfilled only to some extent, as bioreactor design in tissue engineering is very complex and still at an early stage of development. In this review we summarize important aspects for bioreactor design and provide an overview on existing concepts. The generation of three dimensional cartilage-carrier constructs is described to demonstrate how the properties of engineered tissues can be improved significantly by combining biological and engineering knowledge. In the future, a very intimate collaboration between engineers and biologists will lead to an increased fundamental understanding of complex issues that can have an impact on tissue formation in bioreactors.
[Key
References: VOL. 100, 2005 BIOREACTOR DESIGN FOR TISSUE ENGINEERING