Bioplastics

Good Essays
ARTICLE IN PRESS

Biomaterials 24 (2003) 4273–4281

Poly(hydroxybutyrate-co-hydroxyhexanoate) promoted production of extracellular matrix of articular cartilage chondrocytes in vitro
Ying Denga, Xing-Sun Linb, Zhong Zhenga, Jin-Guang Denga, Jin-Chun Chena, Hui Mab, Guo-Qiang Chena,* a Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China b Department of Physics, Tsinghua University, Beijing 100084, China Received 25 November 2002; accepted 13 May 2003

Abstract The present investigation describes the production of extracellular matrix of rabbit articular cartilage chondrocytes grown on scaffolds of polyhydroxybutyrate (PHB) blended with poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) for up to 7 days. The mRNA level of type II collagen of chondrocytes seeded on all scaffolds consisting of PHBHHx were obviously higher than that of PHB-only scaffold throughout the culture period, suggesting the positive effect of PHBHHx on extracellular matrix production. Second-harmonic generation (SHG) imaging technique, combined with confocal fluorescence microscopy (CFM) revealed that PHBHHx in PHB scaffold provided better surface properties for anchoring type II collagen filaments and their penetration into internal layers of the scaffolds. Glycosaminoglycan (GAG), a major composition of extracellular matrix, showed a sharp increase in construct of 1:2 PHB/PHBHHx scaffold after 7 day cultivation, while only a small increase was observed in all other tested scaffolds. At the same time, total collagen contents in all scaffolds containing PHBHHx increased with time, with the maximum collagen production of 742.1799.2 mg/g dry weight observed in construct of 1:2 PHB/PHBHHx scaffold inoculated for 7 days, this was almost 4-fold higher than that in scaffold of PHB only. It appears that the presence of right proportion of PHBHHx in the composite system of PHB/PHBHHx highly favored the production of extracellular matrix of articular



References: [20] [1] Oonishi H, Kushitani S, Yasukawa E, Iwaki H, Hench LL, Wilson J, Tsuji E, Sugihara T. Particulate bioglass compared with ARTICLE IN PRESS Y. Deng et al. / Biomaterials 24 (2003) 4273–4281 [21] Yang X, Zhao K, Chen GQ. Effect of surface treatment on the biocompatibility of microbial polyhydroxyalkanoates. Biomaterials 2002;23(5):1391–7. [22] Deng Y, Zhao K, Zhang XF, Hu P, Chen GQ. Study on the threedimensional proliferation of rabbit articular cartilage-derived chondrocytes on polyhydroxyalkanoate scaffolds. Biomaterials 2002;23(20):4049–56. [23] Campagnola PJ, Millard AC, Terasaki M, Hoppe PE, Malone CJ, Mohler WA. Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues. Biophys J 2002;82(1):493–508. [24] Hile DD, Amirpour ML, Akgerman A, Pishko MV. Active growth factor delivery from poly(d,l-lactide-co-glycolide) foams prepared in supercritical CO(2). J Control Release 2000;66(2–3):177–85. [25] Liang HJ, Tsai CL, Chen PQ, Lu FJ. Oxidative injury induced by synthetic humic acid polymer and monomer in cultured rabbit articular chondrocytes. Life Sci 1999;65(11):1163–73. [26] Ameer GA, Mahmood TA, Langer R. A biodegradable composite scaffold for cell transplantation. J Orthop Res 2002;20(1):16–9. [27] Elisseeff J, McIntosh W, Fu K, Blunk BT, Langer R. Controlledrelease of IGF-I and TGF-beta1 in a photopolymerizing hydrogel for cartilage tissue engineering. J Orthop Res 2001;19(6):1098–104. [28] Saldanha V, Grande DA. Extracellular matrix protein gene expression of bovine chondrocytes cultured on resorbable scaffolds. Biomaterials 2000;21(23):2427–31. [29] Ishizeki K, Kubo M, Yamamoto H, Nawa T. Immunocytochemical expression of type I and type II collagens by rat Meckel’s chondrocytes in culture during phenotypic transformation. Arch Oral Biol 1998;43(2):117–26. [30] Campagnola PJ, Millard AC, Terasaki M, Hoppe PE, Malone CJ, Mohler WA. Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues. Biophys J 2002;82(1 Pt 1):493–508. [31] Grande DA, Halberstadt C, Naughton G, Schwartz R, Manji R. Evaluation of matrix scaffolds for tissue engineering of articular cartilage grafts. J Biomed Mater Res 1997;34(2):211–20. 4281 [32] Freed LE, Vunjak Novakovic G, Biron RJ, Eagles DB, Lesnoy DC, Barlow SK, Langer R. Biodegradable polymer scaffolds for tissue engineering. Biotechnology 1994;12(7):689–93. [33] Frenkel SR, Toolan B, Menche D, Pitman MI, Pachence JM. Chondrocyte transplantation using a collagen bilayer matrix for cartilage repair. J Bone Jt Surg Br 1997;79(5):831–6. [34] Minas T, Nehrer S. Current concepts in the treatment of articular cartilage defects. Orthopedics 1997;20(6):525–38. [35] LeBaron RG, Athanasiou KA. Ex vivo synthesis of articular cartilage. Biomaterials 2000;21(24):2575–87. [36] Lee DA, Bader DL. Compressive strains at physiological frequencies influence the metabolism of chondrocytes seeded in agarose. J Orthop Res 1997;15(2):181–8. [37] Webb K, Hlady V, Tresco PA. Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization. J Biomed Mater Res 1998;41(3):422–30. [38] Daw R, Candan S, Beck AJ, Devlin AJ, Brook IM, MacNeil S, Dawson RA, Short RD. Plasma copolymer surfaces of acrylic acid/1,7 octadiene: surface characterization and the attachment of ROS 17/2.8 osteoblast-like cells. Biomaterials 1998;19(19): 1717–25. [39] Shelton RM, Rasmussen AC, Davies JE. Protein adsorption at the interface between charged polymer substrate and migrating osteoblasts. Biomaterials 1988;9(1):24–9. [40] Van Wachem PB, Hogt AH, Beugeling T, Feijen J, Bantjes A, Detmers JP, Van Aken WG. Adhesion of cultured human endothelial cells onto methacrylate polymers with varying surface wettability and charge. Biomaterials 1987;8(5):323–8. [41] Gauderon R, Lukins PB, Sheppard CJR. Simultaneous multichannel nonlinear imaging: combined two-photon excited fluorescence and second-harmonic generation microscopy. Micron 2001;32(7):685–9. [42] Jian Y, Jian-Zhong B, Shen-Guo W. Enhanced cell affinity of poly (d,l-lactide) by combining plasma treatment with collagen anchorage. Biomaterials 2002;23(12):2607–14.

You May Also Find These Documents Helpful

  • Good Essays

    Bioplastic

    • 1432 Words
    • 6 Pages

    Tabaco National Haigh School Tabaco City UTILIZATION OF PULVERIZED COCOS NUCIFERA FIBER, DRIED EICHHORNIA AND ABELMOSCHUS ESCULENTUS MUCILAGE AS COMPONENTS OF BIOPLASTIC PRODUCT Submitted by: Maria Yvita Queenne Ignacio Aubrey Dacir Katriela Marie de Villa Jolina Mae Rapirap Jhoan Villanueva IV- Newton Introduction The harmful effects of synthetic plastics in the environment have been increasing, resulting to the demand that people explore other means wherein the products can be produced…

    • 1432 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Bioplastic

    • 1706 Words
    • 7 Pages

    Making a plastic from potato starch – extracting starch In this activity you are going to extract starch from potatoes. This starch can be used to make a plastic. A similar process is used in industry to extract starch, which is then used in a number of products, including food and packaging. You will need: q q q q q q Approx 100 g clean (not muddy) potatoes Grater Tea strainer Distilled water Pestle and mortar 100 cm3 measuring cylinder. What to do q q q q q Grate about 100…

    • 1706 Words
    • 7 Pages
    Good Essays
  • Better Essays

    Bioplastics

    • 1241 Words
    • 5 Pages

    BIOPLASTICS Bioplastics are a form of plastics derived from renewable biomass sources, such as vegetable fats and oils, corn starch, pea starch or microbiota. There are a variety of materials bioplastics that can be composed of, including: starches, cellulose, or other biopolymers. APPLICATIONS OF BIOPLASTICS Biodegradable bioplastics are used for disposable items, such as packaging and catering items (crockery, cutlery, pots, bowls and straws). They are also often used for bags, trays, containers…

    • 1241 Words
    • 5 Pages
    Better Essays
  • Good Essays

    Cassava starch as an effective component for Ideal Biodegradable Plastics A Science Investigatory Project presented to Philippine Women’s College of Davao In partial fulfillment Of the requirements in Science and Technology IV (PHYSICS) To Mr. Kenny Lloyd L. Angon Physics Teacher By Gabriel Arañas Danielle Andrea M. Ibaos Jascha Bridget Lim John Vergel Mori Grade 10 – Probity March 2013 Acknowledgement Before I acknowledge the people who supported this Science Investigatory…

    • 1820 Words
    • 8 Pages
    Good Essays
  • Good Essays

    This content of plastic makes it hard to degrade On top of the list of alternatives for plastics that we currently use , is the Bioplastic which is one of many remedies scientists are really looking forward for and relying on. Bioplastics are plastics derived from renewable biomass sources, such as vegetable fats and oils, corn starch, pea starch or microbiota. Bioplastic types such as starch-based plastics or PHA are potentially biodegradable. This feature is directly linked to the chemical structure…

    • 298 Words
    • 2 Pages
    Good Essays
  • Better Essays

    SOUTH AUSTRALIAN MATRICULATION 2CME20 – CHEMISTRY ISSUE INVESTIGATION Should Bioplastic be Used to Replace Petroleum Based Plastic in packaging? Name : Chang Wei Yang Group : G2 Introduction Plastics, which are utilized heavily in packaging from areas such as product wrappers to food wrapping illustrate that versatile plastic is an essential part of our lives. However, with the threat of global warming on the horizon, as well as the depletion of fossil fuels used in the production…

    • 1490 Words
    • 6 Pages
    Better Essays
  • Powerful Essays

    Determination of the tensile strength of starch bioplastic cast-film reinforced with Pineapple peel 1Caburnay, Frances Dianne R., 1Contreras, Jonathan S., 1Dulnuan, Gwen Kristin C 2Asst. Professor Catherine D. Sanchez 1Bachelor of Science in Food Technology 2Chemical Engineer ABSTRACT Natural fibers have become new alternative materials that can replace petroleum-based plastic, either alone or combined with another material, to produce greener composite materials. One of the most important…

    • 2228 Words
    • 9 Pages
    Powerful Essays
  • Powerful Essays

    SALLE GREEN HILLS Comparative Study Between the Bioplastic Properties of Agar-Agar (Gelidium amansii) and Potato (Solanum tuberosum) Starch Submitted by: Kyle Emmanuel A. David Rynno Gabriel Luis T. Garde Justin Carlo P. Gregorio Rufo Angelo M. Mauricio III Christian Michael A. Perreras II-B Submitted to: Miss Alvie Diaz Submitted on: January 30, 2012 ABSTRACT Bioplastic is a form of plastic derived from renewable biomass sources…

    • 2083 Words
    • 9 Pages
    Powerful Essays
  • Good Essays

    Environmental issues of biomass utilisation Biofuels are expected to be produced in larger volumes in the coming decades. However, unlike other renewable energy strategies (solar, wind, sea waves), biomass resources are constrained by the availability of land and water. Energy inputs and fertilizers are also required in their growth, cultivation and production. In some cases, biofuels can consume a significant amount of energy that is derived from fossil fuels (Blottnitz and Curran, 2007).…

    • 387 Words
    • 2 Pages
    Good Essays
  • Satisfactory Essays

    Sambhaji

    • 1232 Words
    • 5 Pages

    PESTEL Analysis Political • Brazilian and Mexican governments should be engaged on the development of incentives to bioplastics manufacturers - especially small and medium sized. The governments, together with industry associations, are expected to be involved in developing standards and certifications for bioplastics, such as the Compostability Regulation. • In Brazil, there exist public programs for recycling plastics (such as a selective collection) and the same model could be applied for compostable…

    • 1232 Words
    • 5 Pages
    Satisfactory Essays