Activity 1.1.5: ELISA
Explain why antibodies allow scientists to target and identify specific disease agents. a. Antibodies allow scientists to target and identify specific disease agents because they bind to antigens to counteract them. The more antigens you have, the more antibodies you have, the more the of the virus/bacteria that there is in you. Why is the secondary antibody used in an ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate? b. Attached to the secondary antibody is an enzyme such as peroxidase or alkaline phosphatase. These enzymes can metabolize colorless substrates (sometimes called chromagens) into colored products. After an incubation period, the secondary antibody solution is removed and loosely adherent ones are washed off as before. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100? c. This means that the concentration of the disease is 1/5. Even furthermore, 1/5 is a bigger ratio than 1/100, meaning that person with a dilution of 1/5 has a higher quantity of the disease in them. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate. d. It is a good idea to complete ELISA assay in triplicate, because by having three samples, a person could have a more conclusive result as to whether the person was positive or negative and the seriousness of their condition. The more samples the merrier. Why do you think college students living in dorms are often populations who see meningitis outbreaks? e. College students living in dorms are often populations who have more meningitis outbreaks, because they stereotypically have a tendency to sleep around since they are in such close proximity of each other. How did...
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