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biology432
7/22/2014

3.01 Cell Cycle Lab Report

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3.01 Cell Cycle Lab Report
Safety Notes:
Always handle microscopes and glass slides carefully.
Wash your hands after handling the prepared specimens.
Materials:
Compound light microscope
Glass microscope slide with prepared onion root tip specimen
Purpose:
understand and identify the stages of the cell cycle and mitosis. apply an analytical technique to estimate the relative length of each stage of the cell cycle.
Hypothesis:
What do you predict you will find about the number of cells in each of the phases of the cell cycle?
Provide an explanation for your prediction.
Procedure:

Data and Observations:
Create a data table containing a tally of the number of cells observed in each of the following stages:

Stage

Number Number of Cells of Cells in Part in Part
1
2

Interphase
Prophase
Metaphase
Anaphase
Telophase
Cytokinesis
Record any observations about the cells you observed (what does the cell look like for each stage):
Data Analysis:
Calculate the percentage of the cell cycle spent in each stage.
Number of cells in given stage ÷ total number of cells counted × 100 = % of the cell cycle spent in this stage
Create a graph that represents the time spent in each stage of the cell cycle.
Conclusion:
Be sure to answer the following reflection questions in the conclusion of your lab report:
Reflection questions need to be answered using complete sentences. Some questions may require a longer explanation in paragraph form.
1. Based on your data, what can you infer about the length of time spent in each stage of the cell cycle?
2. What stages were the longest and shortest? Give a brief explanation of why these stages may have that time period.
3. What is a distinguishing visible feature of each stage of the cell cycle?
4. What differences can you see when you compare the nucleus of a dividing cell with that of a nondividing

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