Design an experiment to investigate the osmosis in plant cells. AIM:
To determine how the osmosis in potato cells measured by change in length of potato cylinders varies with change in concentration of sucrose. MATERIALS REQUIRED:
100ml beakers – 15
The concentration of sucrose solution taken in g dm-3.DEPENDENT VARIABLE: Osmosis of plant cells measured by change in length of potato cylinders.CONTROLLED VARIABLES: Type of potato
A big fresh potato was taken and only cylinders from that potato were obtained. Mass and dimensions of the cylinders
A single cork borer was used to cut out cylinders that were then cut to lengths of 40mm and 52g. Type of solution used
All of the cylinders were immersed in solutions of sucrose and distilled water. Volume of solution
All the cylinders were immersed in beakers containing 40ml of the sucrose solution. Time of immersion
The cylinders were immersed in the solutions for 5hour for osmosis to take place with considerable change in length. Temperature of the osmosis setup
The water was taken from the same source and the entire experiment was conducted at room temperature (25 ̊ C). pH of the solution
Using a pH meter the pH of all the solutions should be determined and it will be approximately neutral since only distilled water and sucrose powder in different concentration have been used PROCEDURE:
Take 24 100ml beakers and divide them into 3 sets.
In each set, name each of the 8 beakers with numbers 20, 25, 30, 35, 40, 45, 50 and 55. These beaker numbers will indicate the concentration of the sucrose solution they will contain. In each beaker add 40ml of distilled water.
Weigh the masses of sucrose powder on the digital balance and mix them to beakers in each set filled with water according to the table shown below- volume of distilled water in cm3...
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