Biology Experiment

Topics: Oxygen, Carbon dioxide, Cellular respiration Pages: 3 (671 words) Published: May 29, 2014
Cell Respiration
Cell respiration refers to the process of converting the chemical energy of organic molecules into a form immediately usable by organisms. Glucose may be oxidized completely if sufficient oxygen is available and is summarized by the following reaction: C6H12O6 + 6 O2(g) 6 H2O + 6 CO2(g) + energy

All organisms, including plants and animals, oxidize glucose for energy. Often, this energy is used to convert ADP and phosphate into ATP. It is known that meal worms undergo cell respiration during germination. Do meal worms undergo cell respiration before germination? Using your collected data, you will be able to answer this question concerning respiration and non-germinated meal worms. Using the O2 Gas Sensor, you will monitor the oxygen consumed by meal worms during cell respiration. Both larval and pupated meal worms will be tested. Additionally, cell respiration of larval meal worms at two different temperatures will be tested.

OBJECTIVES
In this experiment, you will
Use an O2 Gas Sensor to measure concentrations of oxygen gas during cell respiration. Study the effect of temperature on cell respiration rate.
Determine whether meal worms and pupated meal worms respire. Compare the rates of cell respiration in germinating and non-germinating meal worms.

Figure 1

MATERIALS
LabQuest
250 mL respiration chamber
LabQuest App
ice cubes
Vernier O2 Gas Sensor
thermometer
10 meal worm larvae
two 100 mL beakers
10 meal worm pupae
Logger Pro (optional)

PROCEDURE
1.Connect the O2 Gas Sensor to LabQuest and choose New from the File menu. If you have an older sensor that does not auto-ID, manually set up the sensor. 2.On the Meter screen, tap Rate. Change the data-collection rate to 0.1 samples/second and the data-collection length to 600 seconds. 3.Measure the room temperature using a thermometer and record the temperature in Table 1. 4.Obtain 10 meal worm larvae.

5.Place the meal worm larvae into the respiration chamber....
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