Biology
Anisha Malhotra Prof. Lovett Lab Report # 4 Experiment 7&8
1
Bacterial Growth and One Step Burst - T7 Phage
I. INTRODUCTION:
These experiments helped us learn the factors that were involved in the growth of the bacteria that increased our study towards their genetic, physical and metabolic characteristics. We used Escherichia coli and Bacteriophage T7 to identify and analyze their identical life cycle and replication that was involved in their process of growth. As, growth for any bacteria means it multiplying by increasing in its size and quantity, which can easily be examined by measuring its cell mass and cell number. Whereas, Bacteriophage T7 being double stranded makes us analyze the length of its latent period and the average burst size that occurred due to infection over a period of time. In this experiment we formed dilutions and found all the required data to analyze the process that helps in their growth, we look for colonies in Escherichia coli and formation of plaques in Bacteriophage T7. This is one way to uniquely identify them and differ them from one another.
METHOD & MATERIAL:
"As per Lab Manual"
II.
RESULTS: Bacterial Growth Experiment 7 Klett Units Cell # 0 1.88 x 107 1 1.21 x 108 6.0 x 10º 1.36 x 108 2.4 x 10¹ 7.9 x 107 9.0 x 10¹ 5.0 x 107 1.4 x 10² 4.6 x 108 1.4 x 10² 4.6 x 108
Expected Results 2.8 x 107 4.8 x 108 2.2 x 108 8.8 x 108 8.5 x 109 4.0 x 109 4.0 x 109
Time of Incubation 7.5 8 8.5 9 9.5 10 12
Anisha Malhotra Prof. Lovett Lab Report # 4 Experiment 7&8
2
Time In Minutes 0 10 20 30 40 50 60
One Step Burst T7 Phage Experiment 8 Klett Units Plaque # 1 4.8 x 10 ---------1 5.2 x 10 9.0 x 108 5.1 x 101 3.2 x 1011 1 3.8 x 10 7.7 x 1010 2.7 x 101 1.5 x 1010 2.0 x 101 9.1 x 1010 1.0 x 101 3.5 x 1010
Expected Results --------2.0 x 109 7.2 x 109 9.5 x 109 1.5 x 1010 1.4 x 1010 3.5 x 1010
III.
DISCUSSION:
Bacterial Growth of bacteria is easily measured by counting its cell number and cell mass,
1
Bacterial Growth and One Step Burst - T7 Phage
I. INTRODUCTION:
These experiments helped us learn the factors that were involved in the growth of the bacteria that increased our study towards their genetic, physical and metabolic characteristics. We used Escherichia coli and Bacteriophage T7 to identify and analyze their identical life cycle and replication that was involved in their process of growth. As, growth for any bacteria means it multiplying by increasing in its size and quantity, which can easily be examined by measuring its cell mass and cell number. Whereas, Bacteriophage T7 being double stranded makes us analyze the length of its latent period and the average burst size that occurred due to infection over a period of time. In this experiment we formed dilutions and found all the required data to analyze the process that helps in their growth, we look for colonies in Escherichia coli and formation of plaques in Bacteriophage T7. This is one way to uniquely identify them and differ them from one another.
METHOD & MATERIAL:
"As per Lab Manual"
II.
RESULTS: Bacterial Growth Experiment 7 Klett Units Cell # 0 1.88 x 107 1 1.21 x 108 6.0 x 10º 1.36 x 108 2.4 x 10¹ 7.9 x 107 9.0 x 10¹ 5.0 x 107 1.4 x 10² 4.6 x 108 1.4 x 10² 4.6 x 108
Expected Results 2.8 x 107 4.8 x 108 2.2 x 108 8.8 x 108 8.5 x 109 4.0 x 109 4.0 x 109
Time of Incubation 7.5 8 8.5 9 9.5 10 12
Anisha Malhotra Prof. Lovett Lab Report # 4 Experiment 7&8
2
Time In Minutes 0 10 20 30 40 50 60
One Step Burst T7 Phage Experiment 8 Klett Units Plaque # 1 4.8 x 10 ---------1 5.2 x 10 9.0 x 108 5.1 x 101 3.2 x 1011 1 3.8 x 10 7.7 x 1010 2.7 x 101 1.5 x 1010 2.0 x 101 9.1 x 1010 1.0 x 101 3.5 x 1010
Expected Results --------2.0 x 109 7.2 x 109 9.5 x 109 1.5 x 1010 1.4 x 1010 3.5 x 1010
III.
DISCUSSION:
Bacterial Growth of bacteria is easily measured by counting its cell number and cell mass,