biological molecules lab report

Topics: Nutrition, Glucose, Vitamin C Pages: 13 (1086 words) Published: October 24, 2012
Biological Molecules Lab Report

EXERCISES 1 & 2 QUALITATIVE TESTING FOR BIOLOGICAL MOLECULES

1) Complete the table for your group's samples. Be sure to clearly indicate in the blue line whether or not your sample was positive (+) or negative (-) for the given test. Then below that in the white area, type your observations, mainly focusing on the color, but also the layering as appropriate. Be sure your interpretations match your observations! The chart area will expand as you type.

BENEDICT'S TEST FOR SUGAR

IODINE TEST FOR STARCH

BIURET TEST FOR PROTEIN

SUDAN TEST FOR LIPID

WATER (-)

-

-

-

-

_OBSERVATIONS_

Blue

Yellow

Light blue

Clear w/ red layer on top

GLUCOSE (+)

+

-

-

-

_OBSERVATIONS_

Red-Orange

Yellow

Light blue

Clear w/ red layer on top

STARCH (+)

-

+

-

-

_OBSERVATIONS_

Blue

black

Light blue

Clear w/ red layer on top

EGG ALBUMIN (+)

-

-

+

-

_OBSERVATIONS_

blue

yellow

Purple

Clear w/ red layer on top

VEGETABLE OIL (+)

-

-

-

+

_OBSERVATIONS_

blue

yellow

Light blue

Red

RICE MILK

-

-

+

-

_OBSERVATIONS_

Yellow(cloudy)

Yellow(cloudy)

Purple

Clear w/ red layer on top

PEDIALYTE

+

-

-

-

_OBSERVATIONS_

Red-Orange

Yellow

Pink

Yellow w/ red layer

CHICKEN BROTH

+

+

-

-

_OBSERVATIONS_

Red-Orange

Black

Clear

Clear w/ red layer

REGULAR LIME SODA

+

-

-

-

_OBSERVATIONS_

Red-Orange

Red-orange

Clear

Clear w/ red layer

CHAI

-

-

-

-

_OBSERVATIONS_

Green/blue

White(cloudy)

White(cloudy)

White(cloudy)

RED BULL

+

-

-

-

_OBSERVATIONS_

Red-Orange

Red-orange

clear

Yellow w/ red layer

COCONUT WATER

-

-

+

-

_OBSERVATIONS_

Brown(cloudy)

Red-orange

Purple

Clear w/ red layer

BOOST

-

-

-

-

_OBSERVATIONS_

Yellow (separated)

White(cloudy)

Light brown(cloudy)

White (cloudy) w/ red layer on top

2) Write a discussion based on your observations. Analyze each product for the molecules it contains. This does not mean to just restate what was in your sample. Think about it. Analyze it. Does the result make sense, based on the label and on what is known about the food. Do the results make sense to you. If they do not, think about, and research, what might be going on. Do not judge, just report (i.e. "The soda is bad because it is full of sugar" is a judgmental statement).

A series of tests were performed on various different products to determine whether any of them contain glucose, starch, protein, or lipids. The Benedict's test is used to determine whether a product contains glucose. From the products used only Pedialyte, chicken broth, regular lime soda, and red bull tested positive for glucose molecules. When a test is positive it turns into a red-orange color. The results for most of these products make sense with the exception of the pedialyte. At first glance, pedialyte does not seem like it should be in this category. After doing research, it was found that pedialyte is actually made with the amount of glucose and fructose recommended by the American Academy of Pediatrics. Next was the Iodine test for starch. When an Iodine test is positive, it turns black. After conducting this test, it was found that chicken broth was the only product in the list that contains starch. Later the Biuret test was performed to check for protein in products. After conducting the test it was established that rice milk and coconut water both contain protein. At first, this does not seem logical. After doing research it was discovered that rice milk and coconut water do indeed contain protein, although they are not significant amounts....

Cited: "Pedialyte® Liters." _Pedialyte® Liters_. Abbott Nutrition, n.d. Web. 11 Oct. 2012. .
"DRI Tables." _Food and Nutrition_. National Academy of Sciences. Institute of Medicine. Food and Nutrition Board., n.d. Web. 11 Oct. 2012. .
Rowlett, Russ. "USFDA Daily Values." _USFDA Daily Values_. University of North Carolina at Chapel Hill, 19 June 2001. Web. 11 Oct. 2012. .
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