Biochemistry 3

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Topics: DNA, DNA replication
Unit 2 Biosynthesis and Degradation of Nucleotides

Module Description: This module is designed to learn the biosynthesis of nucleotides

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Topics (Chapter 22.4/25.1: Lehninger Principles of Biochemistry) 1. De Novo Purine Nucleotide Synthesis Begins with PRPP 2. Purine Nucleotide Biosynthesis Is Regulated by Feedback Inhibition 3. Pyrimidine Nucleotides Are Made from Aspartate, PRPP, and Carbamoyl Phosphate 4. Pyrimidine Nucleotide Biosynthesis Is Regulated by Feedback Inhibition 5. Nucleoside Monophosphates Are Converted to Nucleoside Triphosphates 6. Ribonucleotides Are the Precursors of Deoxyribonucleotides 7. Thymidylate Is Derived from dCDP and dUMP 8. Degradation of Purines and Pyrimidines Produces Uric Acid and Urea, Respectively 9. Purine and Pyrimidine Bases Are Recycled by Salvage Pathways 10. Excess Uric Acid Causes Gout 11. Many Chemotherapeutic Agents Target Enzymes in the Nucleotide Biosynthetic Pathways 12. DNA Replication Follows a Set of Fundamental Rules 13. DNA Is Degraded by Nucleases 14. DNA Is Synthesized by DNA Polymerases 15. Replication Is Very Accurate 16. E. coli Has at Least Five DNA Polymerases 17. DNA Replication Requires Many Enzymes and Protein Factors 18. Replication of the E. coli Chromosome Proceeds in Stages 19. Bacterial Replication Is Organized in Membrane-Bound Replication Factories 20. Replication in Eukaryotic Cells Is More Complex Unit specific Learning Outcomes  Compare De Novo Purine and Pyrimidine Nucleotide Synthesis  Describe biosynthesis from Nucleoside to Nucleoside Triphosphates  Discuss the conversion of Ribonucleotides to Deoxyribonucleotides  Analyse the biochemical and clinical implication of Degradation of Purines and Pyrimidines  Discuss the biosynthesis of Purine and Pyrimidine by Salvage Pathways  Discuss DNA replication process  Differentiate Eukaryotic and prokaryotic replication Learning Strategy  Interactive group discussion  Pathway animation/video  Seminar/presentation  Independent

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