Biochem
School of Chemical and Life Sciences
Diploma in Biomedical Science
Basic Biochemistry
Formal Report of Experiment 5 & 6:
Introduction to Spectrophotometer in Enzyme Assay & Enzyme Kinetics of beta-galactosidase
Name : Tan Jia Chi Class : DBS / FT/ 1A / 02 Admission no. : 1360084
Content Page
Abstract --------------------------------------------------------------------- 4
Introduction --------------------------------------------------------------------- 5
Procedure ------------------------------------------------------------------- 6
Result ------------------------------------------------------------------- 7-14
Discussions ------------------------------------------------------------------- 15-17
Conclusion ------------------------------------------------------------------- 18
References ------------------------------------------------------------------- 18
3
Abstract
Experiment 5 & 6 are conducted to familiarise the students with the use of spectrophotometer to study the effect of temperature on the enzyme and to study and measure the enzyme activity using spectrophotometer respectively. For Experiment 5, a mixed solution of ONPG solution and Sodium phosphate buffer pH 7.3 is incubate at different temperature, 25, 40, 50, 60 and 70 °C. The mixed solution is then incubated for another 10 minutes after the enzyme is added in their respective temperature. Then, using spectrophotometer, the absorbance in the mixed solution incubated in different temperature is recorded. For Experiment 6, 5 different
Diploma in Biomedical Science
Basic Biochemistry
Formal Report of Experiment 5 & 6:
Introduction to Spectrophotometer in Enzyme Assay & Enzyme Kinetics of beta-galactosidase
Name : Tan Jia Chi Class : DBS / FT/ 1A / 02 Admission no. : 1360084
Content Page
Abstract --------------------------------------------------------------------- 4
Introduction --------------------------------------------------------------------- 5
Procedure ------------------------------------------------------------------- 6
Result ------------------------------------------------------------------- 7-14
Discussions ------------------------------------------------------------------- 15-17
Conclusion ------------------------------------------------------------------- 18
References ------------------------------------------------------------------- 18
3
Abstract
Experiment 5 & 6 are conducted to familiarise the students with the use of spectrophotometer to study the effect of temperature on the enzyme and to study and measure the enzyme activity using spectrophotometer respectively. For Experiment 5, a mixed solution of ONPG solution and Sodium phosphate buffer pH 7.3 is incubate at different temperature, 25, 40, 50, 60 and 70 °C. The mixed solution is then incubated for another 10 minutes after the enzyme is added in their respective temperature. Then, using spectrophotometer, the absorbance in the mixed solution incubated in different temperature is recorded. For Experiment 6, 5 different