BIocem Lab: Measuring Spectra of Methyl Orange and Bromophenol Blue
I. Abstract
The purpose of this practical was to measure the amount of a chemical substance present in a sample. Primarily, the aim of Experiment 1 was to measure the absorption spectrum of a particular coloured substance (in this case Bromophenol Blue and Methyl Orange) at varying wavelengths of light. The goal of this experiment was to measure the given amounts of a given chemical substance that was present in a given sample of Methyl Orange and in blood. We measured the optical density at wavelengths 400 to 700 in intervals of 20 mu. For Experiment 2, the process of the experiment focuses on the substance and records its absorption levels at different concentrations. The purpose of this experiment was for each student to test their blood glucose level and determine if they had low blood glucose, which indicate hypoglycemia, or high blood glucose, which indicate hyperglycemia.
Introduction
II. Introduction
In the first part of the experiment we utilized absorbance measurements using a UV-visible light spectrophotometer to determine the concentrations of a substance. A spectrophotometer consist of a monochromator for the selection of wavelength, a sample holder referred to as a cuvette, a recorder, and a light detector called a phototube. We then went on to measure
The absorption levels (A) that are obtained when samples of any given substance exposed to a source of visible light are measured by: The length (l) of the light path (i.e. the distance over which the light had to travel), and the concentration (c) of that substance. This, essentially, is the Beer-Lambert law. Therefore, a molecule can only absorb a specific amount of visible light because of the size of its container (the length from one side to the other) and the concentration.
Absorption levels vary across compounds. To measure the absorbance, a reference sample has to be used along with the actual dye sample being tested. The intensity of the light passing through the reference cell is
The purpose of this practical was to measure the amount of a chemical substance present in a sample. Primarily, the aim of Experiment 1 was to measure the absorption spectrum of a particular coloured substance (in this case Bromophenol Blue and Methyl Orange) at varying wavelengths of light. The goal of this experiment was to measure the given amounts of a given chemical substance that was present in a given sample of Methyl Orange and in blood. We measured the optical density at wavelengths 400 to 700 in intervals of 20 mu. For Experiment 2, the process of the experiment focuses on the substance and records its absorption levels at different concentrations. The purpose of this experiment was for each student to test their blood glucose level and determine if they had low blood glucose, which indicate hypoglycemia, or high blood glucose, which indicate hyperglycemia.
Introduction
II. Introduction
In the first part of the experiment we utilized absorbance measurements using a UV-visible light spectrophotometer to determine the concentrations of a substance. A spectrophotometer consist of a monochromator for the selection of wavelength, a sample holder referred to as a cuvette, a recorder, and a light detector called a phototube. We then went on to measure
The absorption levels (A) that are obtained when samples of any given substance exposed to a source of visible light are measured by: The length (l) of the light path (i.e. the distance over which the light had to travel), and the concentration (c) of that substance. This, essentially, is the Beer-Lambert law. Therefore, a molecule can only absorb a specific amount of visible light because of the size of its container (the length from one side to the other) and the concentration.
Absorption levels vary across compounds. To measure the absorbance, a reference sample has to be used along with the actual dye sample being tested. The intensity of the light passing through the reference cell is