BIO- substrate concertration
Date: 14th-15th October
Title: The effect of the concentration of hydrogen peroxide on catalase
Aim: to examine how the concentration of the hydrogen peroxide affects the rate of reaction
Hypothesis: The substrate concentration will determine the rate of reaction. The reaction will increase as the concentration increases, but from a certain concentration, the rate will maintain the same rate
Materials:
3% hydrogen peroxide
6% hydrogen peroxide catalase round metal basin measuring cylinder thermometer conical flask rubber stopper and delivery tube
50mL beaker pipettes blue tack stopwatch Method:
1. Refer to apparatus below for set up
2. Pour 10 of 3% hydrogen peroxide into measuring cylinder and pour into beaker. Add 0ml of water to the beaker.
3. Pour substance from beaker into the conical flask
4. Attach rubber stopper with delivery tube to the neck of conical flask
5. Place 2mL of catalase in the pipette and release catalase though rubber stopper. Immediately place blue tack on the opening and start timer.
6. Observe the reaction. Stop timer once water level reaches 20mL
7. Record results
8. Repeat experiment from 2-7 with hydrogen peroxide at 8mL with 2mL of water, 6mL hydrogen peroxide with 4mL of water, 4mL hydrogen peroxide with 6mL water and at 2mL hydrogen peroxide with 8mL water
Risk Assessment:
In this experiment, hydrogen peroxide can be very dangerous and toxic if inhaled or has contact with skin or eyes.
Therefore wearing safety goggles while handling with hydrogen peroxide. Tying up hair and removing any loose clothing such as ties, blazers in which may come into contact with the hydrogen peroxide.
Spillages should also be cleaned up immediately to avoid any accidents and contact of substance to skin.
Results:
3% Hydrogen Peroxide (mL)
Percentage (%)
Time (seconds)
Enzyme Activity
2
0.6
55.23
3.6212
4
1.2
33.06
6.0496
6
1.2
33.06
7.2516
8
2.4
25.28
Title: The effect of the concentration of hydrogen peroxide on catalase
Aim: to examine how the concentration of the hydrogen peroxide affects the rate of reaction
Hypothesis: The substrate concentration will determine the rate of reaction. The reaction will increase as the concentration increases, but from a certain concentration, the rate will maintain the same rate
Materials:
3% hydrogen peroxide
6% hydrogen peroxide catalase round metal basin measuring cylinder thermometer conical flask rubber stopper and delivery tube
50mL beaker pipettes blue tack stopwatch Method:
1. Refer to apparatus below for set up
2. Pour 10 of 3% hydrogen peroxide into measuring cylinder and pour into beaker. Add 0ml of water to the beaker.
3. Pour substance from beaker into the conical flask
4. Attach rubber stopper with delivery tube to the neck of conical flask
5. Place 2mL of catalase in the pipette and release catalase though rubber stopper. Immediately place blue tack on the opening and start timer.
6. Observe the reaction. Stop timer once water level reaches 20mL
7. Record results
8. Repeat experiment from 2-7 with hydrogen peroxide at 8mL with 2mL of water, 6mL hydrogen peroxide with 4mL of water, 4mL hydrogen peroxide with 6mL water and at 2mL hydrogen peroxide with 8mL water
Risk Assessment:
In this experiment, hydrogen peroxide can be very dangerous and toxic if inhaled or has contact with skin or eyes.
Therefore wearing safety goggles while handling with hydrogen peroxide. Tying up hair and removing any loose clothing such as ties, blazers in which may come into contact with the hydrogen peroxide.
Spillages should also be cleaned up immediately to avoid any accidents and contact of substance to skin.
Results:
3% Hydrogen Peroxide (mL)
Percentage (%)
Time (seconds)
Enzyme Activity
2
0.6
55.23
3.6212
4
1.2
33.06
6.0496
6
1.2
33.06
7.2516
8
2.4
25.28