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Bergamot Juice Case Study

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Bergamot Juice Case Study
2.1 The flavonoid-rich fraction of bergamot juice (BJe)
The name of the plant studied in this work “Citrus bergamia Risso & Poiteau” has been checked with The Plant list. This name was in version 1 of The Plant List, (record kew-2724031) and now is a synonym of Citrus limon (L.) Osbeck (http://www.theplantlist.org/tpl1.1/record/tro-28101295).
BJe has been provided by the company "Agrumaria Corleone" (Palermo, Italy) which used fruits of Citrus bergamia Risso & Poiteau from crops located in the province of Reggio Calabria (Italy). BJe was centrifuged at 6000 rpm/min for 15 min to remove impurities and transformed into a dry powder (spray drying method). Aliquots of BJe were stored at -20° C. The drug was defrosted and dissolved in non-pyrogenic saline just prior to use. The chemical profile of flavonoid-rich fraction of BJ has been previously described (Delle Monache et al., …show more content…
Membranes were incubated with peroxidase-conjugated bovine anti-mouse IgG secondary antibody or peroxidase-conjugated goat anti-rabbit IgG (1:2000; Jackson Immuno Research Laboratories, West Grove, PA, USA) for 1 h at room temperature. The levels of β-actin (1:2000;Santa Cruz Biotechnology) and lamin A/C (nuclear fraction 1:500 Sigma-Aldrich, Milan, Italy) served as an internal control for protein loading. The relative expression of the protein bands of IkB-α (37 kDa), NF-κB p65 (65 kDa), iNOS (130 kDa), p-JNK (46 kDa), p-P38(38 kDa), MnSOD (24 Kda) were detected with an enhanced chemiluminescence (ECL) system (Thermo, USA) and visualized with the Chemi Doc XRS (Bio-Rad, USA) and analyzed by using Image Lab 3.0 software (Bio-Rad,
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