Bacteria Morphology LAB2
Bacteria Morphology
April 2, 2015
Abstract – The Purpose of this exercise is to gain experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique.
Hypothesis – The experiment will allow for further insight into stained organisms, allowing extended contrast, differentiating shape and structure through utilization of microscope.
Procedure – Instructions followed as per procedure, specimens viewed through v-scope, using 10x, 20x, and 40x stained wet-mount. Observations compared amongst one another from cd-rom.
Spirillum bacteria
Bacillus
Cheek Swab
Yeast
Analysis – Using the virtual microscope, the images seemed to be more clear and concise. Using the light microscope again for another experiment has increased my confidence in its utilization. Although the procedure was different, the experiment itself was easy to follow. The only challenging aspect of this lab was getting the supplies in order to do the experiment. Fortunately, there were no wait times necessary to view the images, and observation became readily accessible.
Conclusions – The experiment was interesting as the first portion consisted of a virtual microscope, it allowed for a different magnification view and I was able to view these as often as necessary. The other three exercise were not difficult to do, however it was getting the supplies in order for the experiments that I found much difficulty in.
Questions
A. List three reasons why you might choose to stain a particular slide rather than view it as a wet-mount.
Gram stains provide a more intricate view of shape
Gram stains provide better views of structure.
Gram stains show changes in bacteria.
B. Define the following terms:
Chromophore: It is part of the molecule that makes color when light is passed through certain wavelengths
Acidic dye: They are soluble in water possessing an affinity
April 2, 2015
Abstract – The Purpose of this exercise is to gain experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique.
Hypothesis – The experiment will allow for further insight into stained organisms, allowing extended contrast, differentiating shape and structure through utilization of microscope.
Procedure – Instructions followed as per procedure, specimens viewed through v-scope, using 10x, 20x, and 40x stained wet-mount. Observations compared amongst one another from cd-rom.
Spirillum bacteria
Bacillus
Cheek Swab
Yeast
Analysis – Using the virtual microscope, the images seemed to be more clear and concise. Using the light microscope again for another experiment has increased my confidence in its utilization. Although the procedure was different, the experiment itself was easy to follow. The only challenging aspect of this lab was getting the supplies in order to do the experiment. Fortunately, there were no wait times necessary to view the images, and observation became readily accessible.
Conclusions – The experiment was interesting as the first portion consisted of a virtual microscope, it allowed for a different magnification view and I was able to view these as often as necessary. The other three exercise were not difficult to do, however it was getting the supplies in order for the experiments that I found much difficulty in.
Questions
A. List three reasons why you might choose to stain a particular slide rather than view it as a wet-mount.
Gram stains provide a more intricate view of shape
Gram stains provide better views of structure.
Gram stains show changes in bacteria.
B. Define the following terms:
Chromophore: It is part of the molecule that makes color when light is passed through certain wavelengths
Acidic dye: They are soluble in water possessing an affinity