Virtual Bacterial Identification Introduction
KADEN FUNK PERIOD 5
https://www.hhmi.org/biointeractive/bacterial-identification-virtual-labWelcome to the Virtual Bacterial Identification Lab. The purpose of the lab is to familiarize you with the science and techniques used to identify different types of bacteria based on their DNA sequence. Not long ago, DNA sequencing was a time-consuming, tedious process. With readily available commercial equipment and kits, it is now routine. The techniques used in this lab are applicable in a wide variety of settings, including scientific research and forensic labs.
Prepare a sample from a patient and isolate whole bacterial DNA. Make many copies of the desired piece of DNA.
Sequence the DNA.
Analyze the sequence and identify the bacteria.
The piece of DNA used for identifying bacteria is the region that codes for a small subunit of the ribosomal RNA (16S rRNA). We will refer to this piece as 16S rDNA. Different bacterial species have unique 16S rDNA sequences. The identification relies on matching the sequence from your sample against a database of all known 16S rDNA sequences. Learning Objectives
What kind of patient samples are used for the purpose of identifying possible pathogens? Basically any type of bodily fluid. Blood, urine, stool, and lymphnodes.What does PCR do, how does it work, and why is it useful?
The PCR is a process that allows multiplying of DNA with short strands by making billions of copies of such DNA strand through making it by putting it through heat and cool temperatures many times. It heats it to 95 degrees and it is then its chromosomes are cooled, the primers are made softer and combine to the first DNA strand. The DNA polymerase binds and are able to copy each strand of the DNA and depending on the different bacteria the gene will be slightly different. Then because of all the multiple DNA it is then able to be identified. How do you separate the desired DNA from all...
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