Bacillus cereus is a Gram-positive, facultative aerobic, spore-forming, rod-shaped bacterium that is widely distributed among the environment.…
Escherichia coli, more commonly known as E. coli, are a large group of bacteria. It lives in the digestive tracts of humans and also animals. There are many different types, such as enterohemorrhagic E. coli (EHEC) which causes bloody diarrhea. Some types cause urinary tract infections. One other specific type that is real common is called E. coli O157:H7. This type can cause severe anemia, kidney failure, or even death. The way you contract E. coli is coming into contact with feces from humans or animals from food, water, or person to person contact. The most common way to contract E. coli is through food. It can get into meat during processing and if the infected meat is not cooked to 160 degrees Fahrenheit. Any food that has also been in contact with raw meat can also be contaminated. Other foods that can be infected include raw milk and dairy products. The bacteria can spread from the cows udders to the milk. You must make sure everything is pasteurized! Raw fruits and vegetables, especially lettuce, can be infected with animal feces. Unpasteurized apple cider can also put you at risk. Sometimes E. coli gets into water also. Lakes, pools, and water supplies can all be infected. People can become infected when a contaminated city or town water supply has not been properly treated with chlorine or when people accidentally swallow contaminated water while swimming in a lake, pool, or irrigation canal. You can also contract E. coli from person to person contact. This usually happens when people don’t wash their hands after having a bowel movement. The E. coli can spread to another person’s hands or another object. Some people do not notice any symptoms. Children are more likely than adults to have symptoms. Symptoms usually start 3 or 4 days after you come in contact with the E. coli. Some of the most common symptoms are bloody diarrhea, stomach cramps, nausea, and vomiting. Most people get better in about a week and don’t even ever realize that they…
Clostridium Difficile Infection is currently the most commonly found cause of diarrhoea in relation to antibiotic treatments in hospitals. Although C.difficile infections are common, there are only a limited methods of diagnosing accurately if a patient has been affected. I will be discussing methods of diagnosis, pros and cons and statistical facts regarding C.difficile infection.…
Norovirus is contracted through contaminated water and foods, although it can also be spread from person to person as well. Foods that are easily contaminated are shellfish, produce, salads, and any other ready to eat foods frequently touched by food handlers. Water can become contaminated if human waste enters because of flooding or a sewage system not properly working. So, it is very obvious that one can become infected with the norovirus if they consume contaminated food or drink. They can also contract the virus from touching a contaminated surface or object and then directly placing their hands in their mouth.…
C. Diff is passed in feces, spread to food, surfaces and objects when people who are infected don’t wash their hands thoroughly. This bacteria produces spores that can live up to six months on a surface. So if you touch a surface contaminated with C.Diff you can unknowingly ingest the bacteria.…
environment (for example, on clothes, bedding, surfaces, etc) for several months or years. The spores can…
Clostridium Difficile, C. difficile or more commonly called C. diff. belongs to the “domain bacteria which makes it a prokaryotic, its Phylum is Firmicutes, its class is Clostridia, its order is clostridiales, its family is Peptostreptococcaceae” (Brymer). “Clostridium difficile is a spore-forming, Gram-positive anaerobic bacillus that produces two exotoxins: toxin A and toxin B. It is a common cause of antibiotic-associated diarrhea (AAD). It accounts for 15-25% of all episodes of AAD.” [1] The two toxins produced by C. diff are toxin A and toxin B. “Toxin A is an enterotoxin and cytotoxin that causes fluid secretion and increased musical permeability because of the increased inflammation” (Brymer). “Toxin B is a cytotoxin, that bind to…
Bacillus are Prokaryotic cells, that are rod shaped bacteria and can from long chains. Bacillus are also spore forming. When gram staining a Bacillus sample, they are found to be gram positive. However, there are some Bacillus that are gram negative.…
“Frequently Asked Questions about Clostridium difficile for Healthcare Providers.” Healthcare-associated Infections. Centers for Disease Control and Prevention. 25 Nov. 2010. Web. 19 June 2011. <http://www.cdc.gov/HAI/organisms/cdiff/Cdiff_faqs_HCP.html>.…
Clostridium Difficile is a Gram-positive, toxin-producing anaerobic bacterium belonging to the family Clostridiaceae of the Clostridiales. Though strictly oxygen-intolerant, C. Difficile is able to produce aero tolerant endospores under unfavorable conditions that are capable of persisting in an open environment for years. C. Difficile is a commensalist species typically housed in the colonic fecal flora of a fairly small subset of the child population, with the number of carriers decreasing as children age. [1] When it exists in small numbers, this organism remains non-pathogenic. However, when it does manage to colonize and yield larger populations, its pathogenicity becomes the root cause of a variety of colon infections.…
Furthermore, it was through Emile van Ermengem in 1895 whom thoroughly investigated the bacterial agent and toxicologic mechanism of action responsible for botulism that happened during the outbreak in Ellezelles, Belgium. Based from Donald Emmeluth of the book Botulism, it is through the thorough investigation of van Ermengem, he determined that Bacillus botulinus or Clostridium botulinum was the causative agent of food poisoning. The organism’s name was then changed to Clostridium botulinum. Clostridrium Botulinum is described to be a strictly anaerobic gram-positive bacillus bacteria, which means that the bacteria is alive in the absence of oxygen. Moreover, this organism would form endospores when conditions for its growth and survival appear compromised…
Dissecting set was prepared and soaks it in a beaker that contains 70% alcohol. Took a cockle and put it in a dissecting tray. The alcohol was sprayed on the cockle surface. The cockle was opened by using knife. A little tissue was taken from the meat by using inoculation loop. The streak was made on the TSA…
a. The bacteria that causes botulism are found in soil, water, animal guts and dung. When the spores exposed to low oxygen and certain temperature, they produce botulinum toxin, able to germinate, metabolize, food sources through an aerobic respiration to produce toxin. The toxin blocks the release of acetylcholine from motor nerve endings causing paralysis symptoms. The botulism either food born, eating contaminated home-canned foods where the fermentation takes place. or through abrasion in the body skin where the Clostridium bacteria can infect the wound releasing the neurotoxin, this has been increased recently because of the use of tar heroin injection. or infant that he ingests spores and the spores grow in the intestine producing neurotoxin…
Anthrax, also known by its scientific name of Bacillus anthracis, is an infectious disease caused by gram-positive, rod-shaped bacteria. It can produce inactive spores that can live for a long time in the environment, including soil and some animals in the world. Anthrax works when spores get into the body of an animal or human, and then turn into active growing cells.…
When conducting an experiment with microbial organisms there often is change to the bacteria itself and has gone into mutations therefore causing evolution. In the twentieth century there has been many medical breakthroughs. One of those remarkable breakthroughs was Fleming’s discovery of the antibiotic penicillin. That antibiotic had the ability to fight off bacterial diseases and infections through microbial antibacterial chemicals. Through a couple of years the bacteria grew resistant to the penicillin. That was due to gene mutation such as involving genes, which encodes proteins that are capable of deactivating or exporting the antibiotic (Liu, 2012). Over time the bacteria E. coli became resistant to the antibiotics, and once resistant then the medicine (penicillin) has no effect (Doughari 2012). The purpose of the experiment is to understand the processes by which genes can be inserted into plasmids, also to gain some experience in cloning genes via E. coli, lastly to understand the medicinal and other implications of gene transfer among organisms (Hoot, Wimpee, 2012). To test these purposes you would need to gather petri dishes, ampicillin, calcium chloride, and plasmid pJE202.…