Bacillus Badius
The Identification of Bacillus Badius
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Hannah Melanchuk Dr. Robert Suran May 1 2013 Microbiology
Abstract Over a three week time span I was able to successfully identify an unknown microorganism by combining the results of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart, I proceeded to inoculate a Starch plate. In week 2, I analyzed the Starch plate and was able to determine that the results for Starch Hydrolysis were negative due to the dark color. Next, I inoculated Simmons Citrate Agar slants. By week 3 I was able to analyze the Citrate Agar, which tested negative for citrate utilization. With each successive test, the list of possible identities was being lessened until I finally came to the conclusion that only one logical microorganism remained; Bacillus badius.
Introduction In this experiment I am attempting to identify an unknown microorganism. It is important to identify an unknown microorganism for many reasons, such as, if doctors need to treat an infection they need to first identify the bacteria that is causing the infection in order to know how to kill it. Unknown bacteria may also have clinical uses. For example, Penicillin, which is sometimes used to treat ear infections, is actually isolated from a fungus (Types of antibiotics). Microorganisms are classified based on many characteristics and different microorganisms may share several of these characteristics. Thus, making the possibility of identifying the microorganism, using a single test, none. Over a three week time span I was able to successfully identify the organism Bacillus badius by combining the results of several tests. For majority of the procedures I utilized the aseptic technique which consists of sanitary measures, such as, washing your hands. I used the Gram Stain test to determine the organisms’
[pic]
Hannah Melanchuk Dr. Robert Suran May 1 2013 Microbiology
Abstract Over a three week time span I was able to successfully identify an unknown microorganism by combining the results of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart, I proceeded to inoculate a Starch plate. In week 2, I analyzed the Starch plate and was able to determine that the results for Starch Hydrolysis were negative due to the dark color. Next, I inoculated Simmons Citrate Agar slants. By week 3 I was able to analyze the Citrate Agar, which tested negative for citrate utilization. With each successive test, the list of possible identities was being lessened until I finally came to the conclusion that only one logical microorganism remained; Bacillus badius.
Introduction In this experiment I am attempting to identify an unknown microorganism. It is important to identify an unknown microorganism for many reasons, such as, if doctors need to treat an infection they need to first identify the bacteria that is causing the infection in order to know how to kill it. Unknown bacteria may also have clinical uses. For example, Penicillin, which is sometimes used to treat ear infections, is actually isolated from a fungus (Types of antibiotics). Microorganisms are classified based on many characteristics and different microorganisms may share several of these characteristics. Thus, making the possibility of identifying the microorganism, using a single test, none. Over a three week time span I was able to successfully identify the organism Bacillus badius by combining the results of several tests. For majority of the procedures I utilized the aseptic technique which consists of sanitary measures, such as, washing your hands. I used the Gram Stain test to determine the organisms’