Atividade do Extrato de Própolis-Etanólico em Pacientes com Implantes Orais para diminuição de bactérias

Topics: Bacteria, Gram-negative bacteria, Gram-positive bacteria Pages: 8 (4646 words) Published: September 24, 2014
The 2nd Kurdistan Conference on Biological Sciences

J. Duhok Univ. Vol.12, No.1 (Special Issue), Pp 233-239, 2009

University of Duhok 6-8 May, 2008

ANTIMICROBIAL ACTIVITY OF PROPOLIS COLLECTED IN DIFFERENT
REGIONS OF SULAIMANI PROVINCE-KURDISTAN REGION/IRAQ
HASTYAR H. NAJMADEEN and FARHAD A. KH. K AKAMAND
Dept of Biology, College of Science, University of Sulaimani, Kurdistan region, Iraq (Accepted for publication: November 19, 2008)

ABSTRACT
The aim of this study was to investigate the activity of (28%) ethanolic extract propolis (EEP) of [Bazian, Pshdar, Sharbazher, Khormal, Kanakawa and Sulaimani (city center)] against (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aerugenosa, Klebsiella pneumoniae, Proteus mirabilis and yeast Candida albicans). Two methods were employed for propolis activity evaluation; firstly by agar well diffusion method using 40µl of propolis extracts and ethanol 70% as the solvent control per well against tested bacteria and fungus and more commonly used antibiotics were used in comparison to propolis samples. The second method was employed by measuring Minimal Inhibitory Concentration & Minimal Bactericidal; Fungicidal Concentrations (MIC, MBC & MFC) using two fold dilution method for propolis extracts and ethanol 70% as a solvent control in serial concentrations. The Results exhibited that propolis is more efficient than some antibiotic discs. The most sensitive bacteria against (EEP) with mean inhibitory diameters (22-26) mm was S. epidermidis followed by S. aerues and C. albicans (15-22) mm. Tested Gram negative bacteria have lowest inhibitory zones (7-9) mm. Results of MIC & MBC showed S. aureus with (0.1750.7mg/ml) followed by S. epidermidis & C. albicans with (0.7-1.4mg/ml). Among tested Gram negative bacteria, all (EEP) samples showed relatively strong inhibitory action on P. mirabilis (14mg/ml) and weak inhibitory action on P. aerugenosa & K. pneumoniae (28mg/ml) of two of propolis samples. No activity was noticed of solvent control ethanol (70%) neither in the tubes nor in the wells for all tested bacteria and fungus.

KEYWORDS

Propolis

EEP

Antibacterial

Antifungal

INTRODUCTION

P

ropolis(bee glue)is a resinous substance
produced by honeybees. The bees mix exudates
collected from plants with waxes and glandular
secretions to synthesize the substance which is used
for the construction and adaptation of their hives(1, 2).
Bees use propolis as a ‘chemical weapon’ against
pathogenic microorganisms, and it has been used by
humans as a remedy in flok medicine for its health
properties as early in Egypt 3000 BC(1, 3, 4),ancient
Greeks and Romans, etc.(5, 4, 6) and recently in
modern medicine (7).
It is still used for the treatment of various diseases
(diabetes, heart disease and keratitis). It is also used in
the production of health food and(biocosmotics) which
increase formation of antibodies when fed together
with Escherichia coli in rat animal (8, 2, 3, and 5).
Scientific research has revealed its very wide
spectrum of effects, including (antibacterial, antifungal,
antiviral, antiprotozoan,anti-oxidant,anticarcinogenic,
anti-inflammatory, immunomodulatory, and antitumor
agents, wound healing promoters and its use as
anesthetic agent(1, 5,9,10,11,12,13,14). It can cure
minor ulcers in the mouth, angina, as well as skin
infections,therapy of mastitis caused by microorganisms
resistant to antibiotics (8).
Biological properties and chemical compositions
of propolis may vary according to different plant
sources that bees could visit, collecting time and
geographic location (1, 2, 9, 10, and 12). Chemical
analysis revealed that propolis contains more than
200 constitutes (12, 13). Among them are phenolic
compounds predominant including flavonoids as a
major component, cinnamic acids derivatives (3, 12,

MIC

MBC

MFC

13), amino acids, fatty acids, and vitamins like (B1,
B2, B6, C, E),...

References: - Abd El Hady; F. K. and Hegazi,;A. G. (2002). Egyptian Proplois:
2
- Bankova, V. S. ; De Castro, S. L. and Marcucci, M. C. (2000).
- Hegazi,A.G. and Abd El Hady, F. K. (2002). Egyptian Proplois:
3
- Hegazi, A. G. (2004). Antifungal and antibacterial activity of
Egyptian Propolis
- Kosalec, I.; Pepeljnjak,S.;Bakmaz,M., and Vladimir-Knezevic,
S.(2005).Flavonoid analysis and antimicrobial activity of
- Gebara, E. C. E.; Lima, L. A. and Mayer, M. P.A. (2002).
- Oksuz, H.; Duran, N.; Tamer, C.; Cetin, M.; and Silici, S. (2005).
University of Duhok 6-8 May, 2008
Staphyloccucus aureus Keratitis in Rabbits
- Katircioglu, H. and Mercan, N. (2006). Antimicrobial activity and
chemical compositions of Turkish propolis form different
- Teixeira, E. W.;Message,D.; Negri,G.; and Salatino, A. (2006).
- Ansorge, S.; Reinhold, D.; and Lendeckl, U. (2003) Propolis and
some of its constituents down-regulate DNA synthesis and
- Bankova, V. (2000). Determining quality in propolis samples.
- Lisowski, F.(1984). Demystifying health food.Contin.
1- Burdock, G. A. (1998). Review of the biological properties and
toxicity of bee propolis
(2001).The antibacterial activity of propolis produced by Apis
mellifera L.and Brazilian stingless bees,J.Venom.Anim.
- Bankova, V.; De Castro, S. and Marcucci, M. (2000). Propolis:
recent advances in chemistry and plant origin
- Korel, W.; Scheller, S.; Shani, J.; Pietsz, G. and Czuba, Z. (1993).
- Mirzoeva, O.; Grishanin, R. and Calder, P. (1997). Antibacterial
action of propolis and some of its components: the effects of
- Tajaisi-Kikuni, N. and Schilcher, H. (1994). Electron microscopic
and microcalorimetric investigations of the possible
Continue Reading

Please join StudyMode to read the full document

You May Also Find These Documents Helpful

  • Manual de Para Extensao de Linha Pstn Em Aread Rural Essay
  • Essay on Concepto de estrategia
  • Factores de Exito Para Tqm Essay
  • Conformity in De La Salle University Essay
  • Modelos de Toma de Decisiones Essay
  • Definiciones de Metodología de Investigación Essay
  • De-Marketing Essay
  • De Beers Essay

Become a StudyMode Member

Sign Up - It's Free