assessment of family

Topics: Enterobacteria, Gram negative bacteria, Enterobacteriaceae Pages: 3 (383 words) Published: June 29, 2014
POLHN Microbiology

Student Name: SOKOPETI FAKAOSIFOLAU.

Assessment 6 – Gram negative bacilli

1. Which of the following is not true of Enterobacteriaceae? a. Gram negative bacilli
b. Oxidase positive
c. Reduce Nitrate to Nitrite
d. Ferment glucose with acid production

2. Which of the following organisms does not belong to the Enterobacteriaceae group? a. Shigella flexneri
b. Eschericia coli
c. Proteus mirabilis
d. Pseudomonas aeruginosa

3. Name the four species of Shigella.
1. Shigella sonnei
2. Shigella dysenteriae
3. Shigella flexneri
4. Shigella boydii

4. Colonies that appear red with black centres on XLD are likely to be which of the following organisms:- a. Shigella flexneri
b. Escherichia coli
c. Salmonella typhimurium

5. Salmonella, Shigella and Campylobacter are all organisms that usually cause which of the following:- a. Gastroenteritis
b. Meningitis
c. Wound infections

6. Which of the following organisms swarm on Blood agar:-
a. Eschericia coli
b. Proteus mirabilis
c. Pseudomonas aeruginosa

7. Which of the following organisms is usually mucoid:-
a. Eschericia coli
b. Proteus mirabilis
c. Klebsiella pneumoniae

8. Name a selective media useful for the isolation of Vibrio cholerae. (TCBS )Thiosulphate Citrate Bile Salts Sucrose is the media used for isolation of Vibrio cholerae

9. Pseudomonas aeruginosa is an oxidase positive, non-lactose fermenter. True
False

10. For the following Enterobacteriaceae fill in the missing reactions

Urea
ONPG
Lactose
Citrate
Indole
LDC
E. coli
a. -
+
b. +
-
c. +
+
S. typhimurium
d. -
-
e. -
f. -
-
+
S. typhi
-
g. -
-
-
-
h. -
P. mirabilis
i. +
-
j. -
+
k. -
l. -
K. pneumoniae
+ slow
m. +
+
+
n. -
+
Note: Fill in the result beside the letter in the table.

11. A TSI agar slope giving an alkaline (red) slant, acid (yellow) butt with black precipitate is likely to be:- a. Klebsiella...
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