Asaasas Asas
EXPERIMENT NO. 5
LIPIDS
Sheena Grace Abainza (12-8116)
Agatha Abella (12-8046)
Danielle Quigao (12-8051)
Jessa Mae Ramos (12-8049)
February 15, 2013
CHEM42 (Biochemistry) Laboratory
ABSTRACT
This experiment was performed to enlighten the researcher about the different properties and characteristics of lipids including its solubility and its complexity with regards to its components. The test for solubility tagged along the aphorism “like dissolves like” since coconut oil was dissolved in chloroform, ether, and carbon tetrachloride. The emulsification of fats in coconut oil was also conducted and the only the dilute albumin solution showed a positive effect. The saponification reaction was also conducted which is the focal procedure in the production of soap. Cholesterol, a simple lipid, was also tested in different color reactions which yielded to a negative product.
INTRODUCTION
Objectives: 1. To learn the different properties of lipids 2. To know observe the solubility of lipids in polar and nonpolar solvents 3. To know the reaction of coconut oil in litmus paper 4. To form triglycerides from diverse fatty acids through saponification 5. To study the effects of complex lipids in color reactions
Lipids Lipids are found to be water insoluble but they are found to be soluble in fat solvent. They are heterogeneous group of fatty acids. They include fats, oils, waxes and other related substances. They are oily or greasy substances. Thus they are hydrophobic in nature. Proteins, polysaccharides, DNA and RNA are macromolecules. Lipids are not generally classed as macromolecules, even though they share some features of macromolecules. For example: lipids synthesized as linear polymers and they self assemble into larger structures.
Lipids are compounds having the following characteristics:
• They are insoluble in water • Solubility in one or more organic
LIPIDS
Sheena Grace Abainza (12-8116)
Agatha Abella (12-8046)
Danielle Quigao (12-8051)
Jessa Mae Ramos (12-8049)
February 15, 2013
CHEM42 (Biochemistry) Laboratory
ABSTRACT
This experiment was performed to enlighten the researcher about the different properties and characteristics of lipids including its solubility and its complexity with regards to its components. The test for solubility tagged along the aphorism “like dissolves like” since coconut oil was dissolved in chloroform, ether, and carbon tetrachloride. The emulsification of fats in coconut oil was also conducted and the only the dilute albumin solution showed a positive effect. The saponification reaction was also conducted which is the focal procedure in the production of soap. Cholesterol, a simple lipid, was also tested in different color reactions which yielded to a negative product.
INTRODUCTION
Objectives: 1. To learn the different properties of lipids 2. To know observe the solubility of lipids in polar and nonpolar solvents 3. To know the reaction of coconut oil in litmus paper 4. To form triglycerides from diverse fatty acids through saponification 5. To study the effects of complex lipids in color reactions
Lipids Lipids are found to be water insoluble but they are found to be soluble in fat solvent. They are heterogeneous group of fatty acids. They include fats, oils, waxes and other related substances. They are oily or greasy substances. Thus they are hydrophobic in nature. Proteins, polysaccharides, DNA and RNA are macromolecules. Lipids are not generally classed as macromolecules, even though they share some features of macromolecules. For example: lipids synthesized as linear polymers and they self assemble into larger structures.
Lipids are compounds having the following characteristics:
• They are insoluble in water • Solubility in one or more organic
References: 1. Hames, D. and Nigel Hooper. Biochemistry. New York: Taylor and Francis, 2005. 2. Mckee, T. and James R. Mckee. Biochemistry: The Molecular Basis of Life. Boston: McGraw-Hill,2003. 3. Murray, R., Daryl K. Garner and Victor W. Rodwell. Harper’s Illustrated Biochemistry. New York:McGraw-Hill, 2006. 4. Walker, S., and David McMahon. Biochemistry Demystified. New York: McGraw-Hill, 2008. 5. http://en.wikipedia.org/wiki/Lipid 6. http://www2.chemistry.msu.edu/faculty/reusch/VirtTxtJml/lipids.htm 7. http://www.wisc-online.com/Objects/ViewObject.aspx?ID=AP13204