Apush Unit 5 Review

Better Essays
The purpose of this lab was to observe the different amount of distances each DNA samples travel when placed in a gel-electrophoresis box. Restriction endonucleases are critical tools in recombinant DNA methodology. Electrophoresis is the method of determining the size of fragments that are cut by restriction enzymes. These restriction enzymes always cut at their specific protein recognition sites. This is very useful in the sense that no two restriction enzymes codes for exactly the same recognition site, giving it a unique characteristic that is specific for a strand of DNA. Gel electrophoresis is a technique used to separate different sized fragments of DNA or RNA with the use of an electric field. When a molecule enters an electric field, the speed at which the molecule moves is influenced by several factors including: the charge of the molecule, the strength of the electrical field, the size and shape of the molecule, and the density of the medium (in this case the agarose gel) through which the molecule moves. Because of this, scientists are able to separate different groups of DNA or RNA molecules by first positioning all the molecules at a uniform starting point on the agarose gel and then placing the gel in a chamber containing buffer solution and electrodes. A buffer is a solution that adds extra ions to the gel enhancing the conductivity in the agarose gel matrix. Once in the chamber containing buffer solution and electrodes, the molecules of DNA will begin to migrate through the gel and form bands due to the negative charge of the phosphate groups in the backbone of DNA moving towards the positive electrode.

Each band consists of concentrations of homologous DNA molecule fragments which travel at the same rate and are approximately the same size. The amount of time also affects the distance between each band. The longer the amount of time the DNA molecules were electrophoresed, the greater the distance between the bands of DNA fragments because one

You May Also Find These Documents Helpful

  • Good Essays

    Unit 5 Apush study guide

    • 747 Words
    • 3 Pages

    Apush study guide 1. The wilmot proviso stipulated that slavery should be prohibited in the lands acquired as a result of the Mexican War. 2. In its decision in the case of dred scott v. Sanford, the supreme court held that No black slave could be a citizen 3. The whig party turned against president john tyler because 4. In coining the phrase “manifest Destiny” journalist john L. O’Sullivan meant that 5. All of the following were causes of the Mexican war except Mexican desire…

    • 747 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Apush Unit 5 Study Guide

    • 2751 Words
    • 12 Pages

    Populists: Grew from the Farmer’s Alliance Free and unlimited coinage of silver and gold at 16:1 ratio Increased circulating medium (no less that $50 per capita) Graduated income tax It was big in the 1890s (major electoral victories) Several members of the House of Representatives and the election of 1 U.S. senator Against monopolies More Democratic government (more control to people) Wanted more direct government action to help the working class Government ownership of railroads, telegraph…

    • 2751 Words
    • 12 Pages
    Good Essays
  • Satisfactory Essays

    A Summary Of APUSH Review

    • 340 Words
    • 2 Pages

    APUSH Review The following are review questions for first semester. You should plan to research and prepare these essays as part of your semester review. In-class essay writing will occur on December 10 and 12. You will be allowed to bring one page (2 sides) of notes to help you write the essay(s) I select that day. Note sheets will be collected. You can have notes only, no pre-written essays. The best way to prepare is to reread notes, essays, text selections that answer the question.…

    • 340 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    APUSH Unit 7 Review Sheet

    • 3741 Words
    • 15 Pages

    Unit 7 Review Sheet Accomplishments/Failures of Progressive Era: Passed reforms regarding Farmers and the Union workers. BUT no legislation regarding African Americans and racism. TR almost did w/ Booker T but received bad reviews from public. AFL (goals/tactics): Led by Samuel Gompers;
an alliance of skilled workers in craft unions;
concentrated on brea-and-butter issues such as higher wages, shorter hours, and better working conditions. Aligned w/ Democratic Party under Wilson. Alfred Mahan…

    • 3741 Words
    • 15 Pages
    Good Essays
  • Satisfactory Essays

    Apush Review

    • 384 Words
    • 2 Pages

    the model needs to be able to move * The materials need to be something easily accessible and are good for moving around * Needs to show the main idea and concept of a insulin receptor * Can be out of clay or other craft materials 5. – could make the model out of clay or play dough * Needs to show where the two separate places for the insulin and glucose bond and enter the cell * Can move to show how the insulin bonding makes the receptor open to take in glucose 6…

    • 384 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    APUSH EXAM REVIEW

    • 109 Words
    • 1 Page

    APUSH EXAM REVIEW PERIOD 1 Reasons for European Expansion Population grew quickly and land value, prosperity, and trade increased with it. The second change was the rise of nationalism. This new government made the nation more powerful, unified, and imposed new taxes. Also, Muslims began seizing control of routes to Eastern Asia, which led to Europeans to look for a safer and faster route In 1529, King Henry broke his ties with the church. This is when the Puritans separated from the Church of England…

    • 109 Words
    • 1 Page
    Satisfactory Essays
  • Satisfactory Essays

    APUSH Test Review

    • 614 Words
    • 3 Pages

    APUSH Test Review ch.9-15 1. Men took low-paying jobs as factory hands, construction workers, and canal diggers, while the women took positions as domestic servants in middle- and upper-class homes. They were accused of taking jobs and driving down wages. 2. Finney conducted emotional revival meetings that stressed conversion rather than instruction and discipline. 3. Both of the Great Awakenings were sparked by a declining church attendance; both included 'mass preaching', preachers…

    • 614 Words
    • 3 Pages
    Satisfactory Essays
  • Good Essays

    Hsc451 Unit 5 Review Paper

    • 1289 Words
    • 6 Pages

    The staff member is graded on a scale from 1 to 5 where 5 represents outstanding performance. On the 24/11/10 I spoke to Leeann and told her that her appraisal was due and this is the time to review her performance. I then Leeann her appraisal form and explained to her that she was to fill in the first part and hand it back to me in a few days so she can write down how…

    • 1289 Words
    • 6 Pages
    Good Essays
  • Satisfactory Essays

    Chapter 5 APUSH

    • 1029 Words
    • 5 Pages

    Chapter 5 Social • Major population boom! o by 1775 there were 2.5 million people in 13 colonies o youthful and growing quickly • they were slowly moving westward past the Alleghenies • most populous colonies o Virginia o Massachusetts o Pennsylvania o North Carolina o Maryland • Cities o Philadelphia o New York o Boston o Charleston • Many races settled in America o Germans – fled religious persecution, for economy, and fleeing war  mainly in Pennsylvania – Lutheran, very loyal…

    • 1029 Words
    • 5 Pages
    Satisfactory Essays
  • Good Essays

    Apush Dbq 5

    • 689 Words
    • 3 Pages

    AP US History DBQ Essay Even though the loyalist opposed the American Revolution, Patriots wanted to create a nation with equality and a complete different society compared to the Great Britain (Doc B). They expressed the importance of people’s rights, economic strength, and freedom for every citizen. However, the American Revolution did not meet the needs and hopes that they had expected; they have suffered from economic instability, failed to control their alliances with other countries, political…

    • 689 Words
    • 3 Pages
    Good Essays