Antimicrobial Activity of Banana (Musa sapientum Linn.) Inflorescence Ethanolic Extract against E. coli and S. aureus

Topics: Escherichia coli, Bacteria, Antibiotic resistance Pages: 11 (2313 words) Published: January 12, 2014
Department of Education
Agusan National High School
Caraga Administrative Region
Division of Butuan City

Antimicrobial Activity of Banana (Musa sapientum Linn.) Inflorescence Ethanolic Extract against E. coli and S. aureus

Armona Jean B. Ongtico
Mel Algil A. Agra
Jerrson F. Oronan
Researchers’

Mrs. Letecia S. Villanueva
Research Coach

Abstract

The experience of human misery in the form of disease is perhaps as old as the inception of man on the earth. The history of medicine beyond record of human civilization is shrouded in the misery of obscurity; it almost touches the boundaries of mythology, both East and West alike. Several pharmacological industries have evaluated new era for the search of effective antibiotics throughout the world but on the other hand resistance to these an antibiotic by microorganisms has increased. The increasing failure of chemotherapeutics and antibiotic resistance exhibited by pathogenic microbial infectious agents has led to the screening of several medicinal plants for potential antimicrobial activity. They have a long evolution of resistance against microbial agents which has lead to alternative directions in drug development. The development of antibacterial agents derived from micro-organisms and chemotherapeutic agents from plants is a research area of the utmost importance. The present study was designed to evaluate the antimicrobial activity of banana (Musa sapientum Linn.) Blossom extract against Gram-positive and Gram-negative bacteria (Staphylococcus aureus and Escherichia coli). The appropriate extraction process with an outstanding antibacterial activity of the extract was the alcoholic extraction with 80% ethyl alcohol for 48 hours. The antimicrobial activities of the extract were evaluated using paper disc diffusion methods and assay plates. β-sistosterol, 12- hydroxystrearic acid, palmitic acid and d-malic acid and tannic acid, the bioactive compounds isolated from Musa Sapientum Linn. can retain their inhibitory effect against bacterial growth in model media based on the inhibitory zone minimally.

Contents

Page
Title Pagei

Abstractii

Table of Contentsiii

I. Introduction1

II. Materials and Methods

Flow Chart5

Preparation of Dried Samples6

Extraction of Fresh and Dried Banana Inflorescence6

Preparation of 0.5 McFarland Standard7

Preparation of Nutrient Broth and 7
Adjustment of Turbidity

Preparation of Assay Plates and Cotton-Swabbing7

Paper Disc Diffusion Method8

Reading the Assay Plates9

Analyzing the Results9

III. Results and Discussions10

IV. Conclusions and Recommendations13

V. Acknowledgement14

VI. References15

VII. Appendices16

Introduction

The experience of human misery in the form of disease is perhaps as old as the inception of man on the earth. The history of medicine beyond record of human civilization is shrouded in the misery of obscurity; it almost touches the boundaries of mythology, both East and West alike. Human or Animal sacrifices on altars of temples of god was a common practice even during the days when Indus, Nile, and Greek Civilizations were on their climax. Though these acts did not have any direct or otherwise bearing on the health of diseased or wounded, it had its own convincing or satisfying effects. In order to find remedy for illness and for providing relief to the wounded the man discovered its first resort in plants. Several pharmacological industries have evaluated new era for the search of effective antibiotics throughout the world but on the other hand resistance to these an antibiotic by microorganisms has increased. It is known that...

References: Competencies and Training needs of High School Science and Technology; Mr. Jesus D. Nono
(2002)
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