analysis of glucosamine from tablets
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Spectrophotometric Method for Determination of
Tablets
Glucosamine in Tablets
PRIYA GAONKAR, VINEETA KHANVILKAR, RAJANI SHETTIGAR AND CHHAYA GADGOLI*
Saraswathi Vidya Bhavan’s College of Pharmacy, Sonarpada, Dombivli (East)-421 201, India.
A rapid and sensitive method has been developed for the determination of glucosamine sulphate from tablets by
UV-spectrophotometery. In this method, glucosamine sulphate was reacted with phenylisothiocyanate in presence of a base to yield phenylthiourea derivative. This derivative showed maximum absorbance at 240 nm. Beer’s law is obeyed in the concentration range of 5-25 µg/ml. The method was validated in terms of linearity, precision
(relative standard deviation 1.1%), accuracy and specificity. The proposed method is the only method available for spectrophotometric determination of the drug. It is simple, precise, accurate, sensitive and reproducible and can be used for the routine quality control testing of the marketed formulations.
Glucosamine sulphate (GLS) is a water-soluble amine sugar that is extensively used in the treatment of various conditions of arthritis and collagen deficiency 1-4.
Literature survey reveals that the drug can be estimated only by HPLC5 and no spectrophotometric methods have been reported. The present study describes a simple, sensitive, accurate and precise spectrophotometric method for estimation of GLS in tablet formulation.
The reference standard of GLS was procured as a gift sample from M/s Meyer Organics Ltd., Thane.
Phenylisothiocyanate (PITC) AR grade was purchased from Fluka, Switzerland. All the other chemicals and solvents used were of AR grade. UV SL-159 Elico make spectrophotometer was used for the studies.
A standard solution of GLS (1000 µg/ml) was prepared in
0.1 M aqueous sodium acetate solution. (S1). Ten tablets
(Cartilamine®
Spectrophotometric Method for Determination of
Tablets
Glucosamine in Tablets
PRIYA GAONKAR, VINEETA KHANVILKAR, RAJANI SHETTIGAR AND CHHAYA GADGOLI*
Saraswathi Vidya Bhavan’s College of Pharmacy, Sonarpada, Dombivli (East)-421 201, India.
A rapid and sensitive method has been developed for the determination of glucosamine sulphate from tablets by
UV-spectrophotometery. In this method, glucosamine sulphate was reacted with phenylisothiocyanate in presence of a base to yield phenylthiourea derivative. This derivative showed maximum absorbance at 240 nm. Beer’s law is obeyed in the concentration range of 5-25 µg/ml. The method was validated in terms of linearity, precision
(relative standard deviation 1.1%), accuracy and specificity. The proposed method is the only method available for spectrophotometric determination of the drug. It is simple, precise, accurate, sensitive and reproducible and can be used for the routine quality control testing of the marketed formulations.
Glucosamine sulphate (GLS) is a water-soluble amine sugar that is extensively used in the treatment of various conditions of arthritis and collagen deficiency 1-4.
Literature survey reveals that the drug can be estimated only by HPLC5 and no spectrophotometric methods have been reported. The present study describes a simple, sensitive, accurate and precise spectrophotometric method for estimation of GLS in tablet formulation.
The reference standard of GLS was procured as a gift sample from M/s Meyer Organics Ltd., Thane.
Phenylisothiocyanate (PITC) AR grade was purchased from Fluka, Switzerland. All the other chemicals and solvents used were of AR grade. UV SL-159 Elico make spectrophotometer was used for the studies.
A standard solution of GLS (1000 µg/ml) was prepared in
0.1 M aqueous sodium acetate solution. (S1). Ten tablets
(Cartilamine®
References: Rheumatol., 1995, 22, 201. Lohmander, L.S. and Felson, D.T., J. Rheumatol., 1997, 24,782. Liang, M.H., Pillemer, S.R., Steen, V.D. and Wolfe, F., Arthritis Rheum., 1998, 41, 778. Foley, C.M. and Kratz, A.M., J. Amer. Nutraceutical Assn., 1999, 2, 6. Indian J. Pharm. Sci., 2006, 68 (1): 83-84 January - February 2006