Analysis Of Azadirachtaindica
Abstract— Azadirachtaindica fruit pulp and leaf extract were used to test antimicrobial activity against disease causing bacteria Staphylococcuaureus. Extracts of varying concentrations of Azadirachtaindica fruit pulp and leaf extract were prepared using Phosphate Buffer and tested against test organisms using agar diffusion method. Oxfloxacinof same varying concentrations were used to compare the effect of antimicrobial activity of fruit pulp and leaf extract.
Keywords: Azadirachtaindica, Antimicrobial activity, Staphylococcus aureus.
I. INTRODUCTION
Azadirachtaindica, also known as Neem, is a tree in the mahogany family Meliaceae. It is one of two species in the genus Azadirachta, and is native to India and the Indian subcontinent including Nepal, Pakistan, Bangladesh and Sri Lanka. It is typically grown in tropical and semi-tropical regions. Its fruits and seeds are the source of neem oil. Neem is a fast-growing tree that can reach a height of 15–20 metres (49–66 ft), and rarely 35–40 metres (115–131 ft). It is evergreen, but in severe drought it may shed most or nearly all of its leaves. The branches are …show more content…
When well is loaded with antibiotics, it diffuses in the medium and inhibits the growth of organism. There is logarithmic reduction in antibiotic concentration. The zone of inhibition of bacterial growth around each well is measured and the susceptibility is determined. Medium-Muller Hinton Agar (3.8gm/100ml of distilled water) was prepared, autoclaved at 121⁰ C for 15minutes at 15lbs and poured in sterile petri plates up to a uniform thickness of approximately 5-6mm and the agar was allowed to set at ambient temperature and used. Inoculums-The microorganisms were inoculated in Nutrient broth and incubated at 37⁰ C and were used as inoculums. 25 µl of inoculum was spread over the MHA medium, using sterile
Keywords: Azadirachtaindica, Antimicrobial activity, Staphylococcus aureus.
I. INTRODUCTION
Azadirachtaindica, also known as Neem, is a tree in the mahogany family Meliaceae. It is one of two species in the genus Azadirachta, and is native to India and the Indian subcontinent including Nepal, Pakistan, Bangladesh and Sri Lanka. It is typically grown in tropical and semi-tropical regions. Its fruits and seeds are the source of neem oil. Neem is a fast-growing tree that can reach a height of 15–20 metres (49–66 ft), and rarely 35–40 metres (115–131 ft). It is evergreen, but in severe drought it may shed most or nearly all of its leaves. The branches are …show more content…
When well is loaded with antibiotics, it diffuses in the medium and inhibits the growth of organism. There is logarithmic reduction in antibiotic concentration. The zone of inhibition of bacterial growth around each well is measured and the susceptibility is determined. Medium-Muller Hinton Agar (3.8gm/100ml of distilled water) was prepared, autoclaved at 121⁰ C for 15minutes at 15lbs and poured in sterile petri plates up to a uniform thickness of approximately 5-6mm and the agar was allowed to set at ambient temperature and used. Inoculums-The microorganisms were inoculated in Nutrient broth and incubated at 37⁰ C and were used as inoculums. 25 µl of inoculum was spread over the MHA medium, using sterile